X0306 4 2022-06-07 0 0001636282 Aeglea BioTherapeutics, Inc. AGLE 0001656151 Shanafelt Armen C/O AEGLEA BIOTHERAPEUTICS, INC. 805 LAS CIMAS PARKWAY, SUITE 100 AUSTIN TX 78746 1 0 0 0 Director Stock Option (right to buy) 0.73 2022-06-07 4 A 0 43000 0.00 A 2032-06-06 Common Stock 43000 43000 D The stock option vests and becomes exercisable in 12 equal monthly installments beginning on July 7, 2022, or on the date of the next annual meeting of stockholders, if earlier, subject to the continuing service of the Reporting Person on each vesting date. /s/ James Kastenmayer, by power of attorney 2022-06-09