<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3</documentType>

    <periodOfReport>2016-02-29</periodOfReport>

    <noSecuritiesOwned>0</noSecuritiesOwned>

    <issuer>
        <issuerCik>0001653821</issuerCik>
        <issuerName>CEN BIOTECH INC</issuerName>
        <issuerTradingSymbol>CENBF</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001736938</rptOwnerCik>
            <rptOwnerName>Chaaban Bahige B.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>300-3295 QUALITY WAY</rptOwnerStreet1>
            <rptOwnerStreet2></rptOwnerStreet2>
            <rptOwnerCity>WINDSOR, ONTARIO, CANADA</rptOwnerCity>
            <rptOwnerState>A6</rptOwnerState>
            <rptOwnerZipCode>N8T 3R9</rptOwnerZipCode>
            <rptOwnerStateDescription>ONTARIO, CANADA</rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>1</isDirector>
            <isOfficer>1</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <officerTitle>Chief Executive Officer</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Common Stock</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>144793</value>
                    <footnoteId id="F1"/>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">Bahige (Bill) Chaaban (the &quot;Reporting Person&quot;) was appointed to his positions with CEN Biotech, Inc. (the &quot;Issuer&quot;) on February 29, 2016 and owned 144,793 shares of the Issuer's common stock on such date. The Reporting Person resigned as the Chief Executive Officer of the Issuer on July 12, 2017 and served as the President of the Issuer beginning on July 12, 2017, and served as the Interim Chief Executive Officer of the Issuer from November 13, 2019 until April 18, 2021 and was appointed as the Chief Executive Officer of the Issuer on April 19, 2021.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ Bahige Chaaban</signatureName>
        <signatureDate>2021-04-22</signatureDate>
    </ownerSignature>
</ownershipDocument>
