<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3</documentType>

    <periodOfReport>2017-07-12</periodOfReport>

    <noSecuritiesOwned>0</noSecuritiesOwned>

    <issuer>
        <issuerCik>0001653821</issuerCik>
        <issuerName>CEN BIOTECH INC</issuerName>
        <issuerTradingSymbol>CENBF</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001736911</rptOwnerCik>
            <rptOwnerName>Byrne Joseph A.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>300-3295 QUALITY WAY</rptOwnerStreet1>
            <rptOwnerStreet2></rptOwnerStreet2>
            <rptOwnerCity>WINDSOR, ONTARIO, CANADA</rptOwnerCity>
            <rptOwnerState>A6</rptOwnerState>
            <rptOwnerZipCode>N8T 3R9</rptOwnerZipCode>
            <rptOwnerStateDescription>ONTARIO, CANADA</rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>1</isDirector>
            <isOfficer>1</isOfficer>
            <officerTitle>Chief Executive Officer</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Common Stock</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>12894</value>
                    <footnoteId id="F1"/>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
    </nonDerivativeTable>

    <derivativeTable>
        <derivativeHolding>
            <securityTitle>
                <value>Convertible Note</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>1.6000</value>
            </conversionOrExercisePrice>
            <exerciseDate>
                <value>2016-08-17</value>
            </exerciseDate>
            <expirationDate>
                <value>2020-08-17</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>76123</value>
                    <footnoteId id="F2"/>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeHolding>
    </derivativeTable>

    <footnotes>
        <footnote id="F1">Joseph Byrne (the &quot;Reporting Person&quot;) was appointed to his positions with CEN Biotech, Inc. (the &quot;Issuer&quot;) on July 12, 2017 and owned 12,894 shares of the Issuer's common stock at such date. The Reporting Person resigned from all positions with the Issuer on November 13, 2019. The Reporting Person was appointed as President and a member of the Board of the Issuer effective April 19, 2021.</footnote>
        <footnote id="F2">The Reporting Person also beneficially owned, on July 12, 2017, a convertible note with 76,123 shares issuable upon conversion of same.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ Joseph Byrne</signatureName>
        <signatureDate>2021-04-22</signatureDate>
    </ownerSignature>
</ownershipDocument>
