SCHEDULE 13G/A 0001104659-24-111584 0000901832 XXXXXXXX LIVE 1 Common Stock, $0.001 par value per share 12/31/2025 0001645469 Monopar Therapeutics Inc. 61023L207 1000 Skokie Blvd Suite 350 Wilmette IL 60091 Rule 13d-1(c) AstraZeneca PLC b X0 522667.00 0.00 522667.00 0.00 522667.00 N 7.8 CO Note to Rows 5, 7 and 9: Represents shares directly held by Alexion Pharmaceuticals, Inc., a wholly-owned subsidiary of AstraZeneca PLC. AstraZeneca PLC and Alexion Pharmaceuticals, Inc. may each be deemed to have sole voting and dispositive power over the shares. Note to Row 11: Based upon 6,682,584 shares of Common Stock of the Issuer outstanding as of October 31, 2025, as reported in the Issuer's Quarterly Report on Form 10-Q ("Form 10-Q") filed with the Securities and Exchange Commission (the "SEC") on November 13, 2025. Alexion Pharmaceuticals, Inc. b DE 522667.00 0.00 522667.00 0.00 522667.00 N 7.8 CO Note to Rows 5, 7 and 9: Represents shares directly held by Alexion Pharmaceuticals, Inc., a wholly-owned subsidiary of AstraZeneca PLC. AstraZeneca PLC and Alexion Pharmaceuticals, Inc. may each be deemed to have sole voting and dispositive power over the shares. Note to Row 11: Based upon 6,682,584 shares of Common Stock of the Issuer outstanding as of October 31, 2025, as reported in the Issuer's Form 10-Q filed with the SEC on November 13, 2025. Monopar Therapeutics Inc. 1000 Skokie Blvd., Suite 350 Wilmette, IL, 60091 See response to Item 2(c). See response to Item 2(c). This statement is filed on behalf of: AstraZeneca PLC 1 Francis Crick Avenue Cambridge Biomedical Campus Cambridge CB2 0AA Citizenship: United Kingdom Alexion Pharmaceuticals, Inc. 121 Seaport Boulevard Boston, Massachusetts 02210 Citizenship: United States of America Y 522,667 shares 7.8%. Based upon 6,682,584 shares of Common Stock of the Issuer outstanding as of October 31, 2025, as reported in the Issuer's Form 10-Q filed with the SEC on November 13, 2025. AstraZeneca PLC: 522,667 shares Alexion Pharmaceuticals, Inc.: 522,667 shares AstraZeneca PLC: 0 shares Alexion Pharmaceuticals, Inc.: 0 shares AstraZeneca PLC: 522,667 shares Alexion Pharmaceuticals, Inc.: 522,667 shares AstraZeneca PLC: 0 shares Alexion Pharmaceuticals, Inc.: 0 shares Y Y Y Y Y N By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under ?? 240.14a-11. AstraZeneca PLC /s/ Matthew Bowden 02/06/2026 Alexion Pharmaceuticals, Inc. /s/ Todd Spalding 02/06/2026