AIT Therapeutics, Inc.

2 Ilan Ramon, Science Park

Ness Ziona, 7403635 Israel

VIA EDGAR

Securities and Exchange Commission

Division of Corporation Finance

100 F Street, N.E.

Washington, D.C.  20549

Attention:        Andri Carpenter

Gary Todd

Heather Percival

Geoff Kruczek

Amanda Ravitz

Re:          AIT Therapeutics, Inc.

Registration Statement on Form S-1, as amended

File No. 333-216287

Ladies and Gentlemen:

AIT Therapeutics, Inc., a Delaware corporation (the "Company"), hereby requests acceleration of the effective date of the above-referenced Registration Statement on Form S-1 (as amended, the "Registration Statement") so that such Registration Statement shall become effective at 4:00 p.m. (Washington, D.C. time) on Friday, May 26, 2017 or as soon thereafter as practicable.

Please provide a copy of the Commission's order declaring the Registration Statement effective to Amir Avniel at the address first set forth above.

Respectfully yours,

AIT Therapeutics, Inc.

By: /s/ Amir Avniel

Amir Avniel

President, Chief Executive Officer, and Director