X0407 4 2023-06-12 0 0001641281 Bolt Biotherapeutics, Inc. BOLT 0001415015 O'CALLAGHAN BRIAN C/O BOLT BIOTHERAPEUTICS, INC. 900 CHESAPEAKE DRIVE REDWOOD CITY CA 94063 1 0 0 0 0 Stock Option (Right to Buy) 1.63 2023-06-12 4 A 0 25000 0.00 A 2033-06-11 Common Stock 25000 25000 D The shares subject to the option will vest on the earlier of June 12, 2024 or the day immediately prior to the next annual meeting of stockholders, subject to the Reporting Person's continuous service through such date; provided, however that the option will vest in full upon a change in control of the Issuer. /s/ William P. Quinn, Attorney-in-Fact 2023-06-14