<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3</documentType>

    <periodOfReport>2021-05-19</periodOfReport>

    <noSecuritiesOwned>0</noSecuritiesOwned>

    <issuer>
        <issuerCik>0001639825</issuerCik>
        <issuerName>PELOTON INTERACTIVE, INC.</issuerName>
        <issuerTradingSymbol>PTON</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001865163</rptOwnerCik>
            <rptOwnerName>TCV IX (A) Opportunities, L.P.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O TCV</rptOwnerStreet1>
            <rptOwnerStreet2>250 MIDDLEFIELD ROAD</rptOwnerStreet2>
            <rptOwnerCity>MENLO PARK</rptOwnerCity>
            <rptOwnerState>CA</rptOwnerState>
            <rptOwnerZipCode>94025</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isOther>true</isOther>
            <otherText>May be part of a 13(d) group</otherText>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001666760</rptOwnerCik>
            <rptOwnerName>TCV IX (B), L.P.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O TCV</rptOwnerStreet1>
            <rptOwnerStreet2>250 MIDDLEFIELD ROAD</rptOwnerStreet2>
            <rptOwnerCity>MENLO PARK</rptOwnerCity>
            <rptOwnerState>CA</rptOwnerState>
            <rptOwnerZipCode>94025</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isOther>true</isOther>
            <otherText>May be part of a 13(d) group</otherText>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001361345</rptOwnerCik>
            <rptOwnerName>TCV Member Fund, L.P.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O TCV</rptOwnerStreet1>
            <rptOwnerStreet2>250 MIDDLEFIELD ROAD</rptOwnerStreet2>
            <rptOwnerCity>MENLO PARK</rptOwnerCity>
            <rptOwnerState>CA</rptOwnerState>
            <rptOwnerZipCode>94025</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isOther>true</isOther>
            <otherText>May be part of a 13(d) group</otherText>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001865199</rptOwnerCik>
            <rptOwnerName>TCV X (A) Blocker, L.P.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O TCV</rptOwnerStreet1>
            <rptOwnerStreet2>250 MIDDLEFIELD ROAD</rptOwnerStreet2>
            <rptOwnerCity>MENLO PARK</rptOwnerCity>
            <rptOwnerState>CA</rptOwnerState>
            <rptOwnerZipCode>94025</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isOther>true</isOther>
            <otherText>May be part of a 13(d) group</otherText>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001749363</rptOwnerCik>
            <rptOwnerName>TCV X (B), L.P.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O TCV</rptOwnerStreet1>
            <rptOwnerStreet2>250 MIDDLEFIELD ROAD</rptOwnerStreet2>
            <rptOwnerCity>MENLO PARK</rptOwnerCity>
            <rptOwnerState>CA</rptOwnerState>
            <rptOwnerZipCode>94025</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isOther>true</isOther>
            <otherText>May be part of a 13(d) group</otherText>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001786709</rptOwnerCik>
            <rptOwnerName>TCV X Member Fund, L.P.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O TCV</rptOwnerStreet1>
            <rptOwnerStreet2>250 MIDDLEFIELD ROAD</rptOwnerStreet2>
            <rptOwnerCity>MENLO PARK</rptOwnerCity>
            <rptOwnerState>CA</rptOwnerState>
            <rptOwnerZipCode>94025</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isOther>true</isOther>
            <otherText>May be part of a 13(d) group</otherText>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Class A Common Stock</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>8560</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                    <footnoteId id="F1"/>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Class A Common Stock</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>1620</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>TCV IX (B), L.P.</value>
                    <footnoteId id="F2"/>
                </natureOfOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Class A Common Stock</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>2339</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>TCV Member Fund, L.P.</value>
                    <footnoteId id="F3"/>
                </natureOfOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Class A Common Stock</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>3290</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>TCV X (A) Blocker, L.P.</value>
                    <footnoteId id="F4"/>
                </natureOfOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Class A Common Stock</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>647</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>TCV X (B), L.P.</value>
                    <footnoteId id="F5"/>
                </natureOfOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Class A Common Stock</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>739</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>TCV X Member Fund, L.P.</value>
                    <footnoteId id="F6"/>
                </natureOfOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">These shares are directly held by TCV IX (A) Opportunities, L.P.</footnote>
        <footnote id="F2">These shares are directly held by TCV IX (B), L.P.</footnote>
        <footnote id="F3">These shares are directly held by TCV Member Fund, L.P.</footnote>
        <footnote id="F4">These shares are directly held by TCV X (A) Blocker, L.P.</footnote>
        <footnote id="F5">These shares are directly held by TCV X (B), L.P.</footnote>
        <footnote id="F6">These shares are directly held by TCV X Member Fund, L.P.</footnote>
    </footnotes>

    <remarks>This Form 3 is filed by more than one Reporting Person and is a joint filing by TCV IX (A) Opportunities, L.P., TCV IX (B), L.P., TCV Member Fund, L.P., TCV X (A) Blocker, L.P., TCV X (B), L.P. and TCV X Member Fund, L.P. (collectively, the &quot;Reporting Entities&quot;). The Reporting Entities first acquired such shares of Class A common stock of the issuer on May 19, 2021 and may be deemed part of a Section 13(d) group.</remarks>

    <ownerSignature>
        <signatureName>By: /s/ Frederic D. Fenton, Authorized Signatory for TCV IX (A) Opportunities, L.P.</signatureName>
        <signatureDate>2021-06-01</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>By: /s/ Frederic D. Fenton, Authorized Signatory for TCV IX (B), L.P.</signatureName>
        <signatureDate>2021-06-01</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>By: /s/ Frederic D. Fenton, Authorized Signatory for TCV Member Fund, L.P.</signatureName>
        <signatureDate>2021-06-01</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>By: /s/ Frederic D. Fenton, Authorized Signatory for TCV X (A) Blocker, L.P.</signatureName>
        <signatureDate>2021-06-01</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>By: /s/ Frederic D. Fenton, Authorized Signatory for TCV X (B), L.P.</signatureName>
        <signatureDate>2021-06-01</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>By: /s/ Frederic D. Fenton, Authorized Signatory for TCV X Member Fund, L.P.</signatureName>
        <signatureDate>2021-06-01</signatureDate>
    </ownerSignature>
</ownershipDocument>
