144 0001853327 XXXXXXXX LIVE 0001636282 SPYRE THERAPEUTICS, INC. 001-37722 221 Crescent Street Building 23 Suite 105 Waltham MA 02453 617-651-5940 SCOTT BURROWS Officer Common Morgan Stanley Smith Barney LLC Executive Financial Services

1 New York Plaza 8th Floor New York NY 10004

27500 2047375.00 78540164 05/01/2026 NASDAQ Common 05/01/2026 Exercise of Stock Options Issuer N 27500 05/01/2026 Cash N 10b5-1 Sales for SCOTT BURROWS

221 Crescent Street Building 23 Suite 105 Waltham MA 02453

Common 04/01/2026 7500 370066.25 10b5-1 Sales for SCOTT BURROWS

221 Crescent Street Building 23 Suite 105 Waltham MA 02453

Common 03/03/2026 2500 101620.50 The securities to be sold were and will be received upon the exercise of stock options over the next three months. The dates of acquisition and payment were and will be the dates of exercise/sale. 05/01/2026 11/10/2025 /s/ Scott Burrows