Exhibit 21.1

Subsidiaries of Spyre Therapeutics, Inc.

Name of Subsidiary	Jurisdiction
Spyre Therapeutics LLC	Delaware
Aeglea Development Company, Inc.	Delaware
AERase, Inc.	Delaware
AECase, Inc.	Delaware
AEMase, Inc.	Delaware
AE4ase, Inc.	Delaware
AE5ase, Inc.	Delaware
AE6ase, Inc.	Delaware
Aeglea Biotherapeutics UK Limited	England and Wales
Aeglea Ireland Limited	Ireland
Aeglea BioTherapeutics US LLC	Delaware

         DOCPROPERTY "DOCID" \* MERGEFORMAT