<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0707</schemaVersion>

    <submissionType>D</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001632221</cik>
        <entityName>2401 PENN AVENUE REIT, LLC</entityName>
        <issuerAddress>
            <street1>801 GRAND AVENUE, SUITE 2800</street1>
            <city>DES MOINES</city>
            <stateOrCountry>IA</stateOrCountry>
            <stateOrCountryDescription>IOWA</stateOrCountryDescription>
            <zipCode>50309</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>(515) 247-5767</issuerPhoneNumber>
        <jurisdictionOfInc>DELAWARE</jurisdictionOfInc>
        <issuerPreviousNameList>
            <value>None</value>
        </issuerPreviousNameList>
        <edgarPreviousNameList>
            <value>None</value>
        </edgarPreviousNameList>
        <entityType>Limited Liability Company</entityType>
        <yearOfInc>
            <withinFiveYears>true</withinFiveYears>
            <value>2014</value>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>NA</firstName>
                <lastName>PRINCIPAL ENHANCED PROPERTY FUND, L.P.</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>801 GRAND AVENUE, SUITE 2800</street1>
                <city>DES MOINES</city>
                <stateOrCountry>IA</stateOrCountry>
                <stateOrCountryDescription>IOWA</stateOrCountryDescription>
                <zipCode>50309</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Promoter</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>MANAGER OF THE ISSUER</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>NA</firstName>
                <lastName>PRINCIPAL ENHANCED PROPERTY FUND GP, LLC</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>801 GRAND AVENUE, SUITE 2800</street1>
                <city>DES MOINES</city>
                <stateOrCountry>IA</stateOrCountry>
                <stateOrCountryDescription>IOWA</stateOrCountryDescription>
                <zipCode>50309</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Promoter</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>GENERAL PARTNER OF THE MANAGER OF THE ISSUER</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>NA</firstName>
                <lastName>PRINCIPAL REAL ESTATE INVESTORS, LLC</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>801 GRAND AVENUE, SUITE 2800</street1>
                <city>DES MOINES</city>
                <stateOrCountry>IA</stateOrCountry>
                <stateOrCountryDescription>IOWA</stateOrCountryDescription>
                <zipCode>50309</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Promoter</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>SOLE MEMBER OF THE GENERAL PARTNER OF THE MANAGER OF THE ISSUER</relationshipClarification>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>REITS and Finance</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>Decline to Disclose</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06b</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>false</isAmendment>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2015-01-21</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>false</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isEquityType>true</isEquityType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
            <clarificationOfResponse></clarificationOfResponse>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>500</minimumInvestmentAccepted>
        <salesCompensationList>
            <recipient>
                <recipientName>CHARLES B. HARRISON</recipientName>
                <recipientCRDNumber>3071551</recipientCRDNumber>
                <associatedBDName>H&amp;L EQUITIES, LLC</associatedBDName>
                <associatedBDCRDNumber>113794</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>1175 PEACHTREE STREET, NE, SUITE 2200</street1>
                    <city>ATLANTA</city>
                    <stateOrCountry>GA</stateOrCountry>
                    <stateOrCountryDescription>GEORGIA</stateOrCountryDescription>
                    <zipCode>30361</zipCode>
                </recipientAddress>
                <statesOfSolicitationList>
                    <state>GA</state>
                    <description>GEORGIA</description>
                    <state>TX</state>
                    <description>TEXAS</description>
                    <state>FL</state>
                    <description>FLORIDA</description>
                </statesOfSolicitationList>
                <foreignSolicitation>false</foreignSolicitation>
            </recipient>
            <recipient>
                <recipientName>PHYLLIS J. BECK</recipientName>
                <recipientCRDNumber>1020488</recipientCRDNumber>
                <associatedBDName>H&amp;L EQUITIES, LLC</associatedBDName>
                <associatedBDCRDNumber>113794</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>1175 PEACHTREE STREET, NE, SUITE 2200</street1>
                    <city>ATLANTA</city>
                    <stateOrCountry>GA</stateOrCountry>
                    <stateOrCountryDescription>GEORGIA</stateOrCountryDescription>
                    <zipCode>30361</zipCode>
                </recipientAddress>
                <statesOfSolicitationList>
                    <state>GA</state>
                    <description>GEORGIA</description>
                    <state>TX</state>
                    <description>TEXAS</description>
                </statesOfSolicitationList>
                <foreignSolicitation>false</foreignSolicitation>
            </recipient>
            <recipient>
                <recipientName>SCOTT HARRISON</recipientName>
                <recipientCRDNumber>4981070</recipientCRDNumber>
                <associatedBDName>H&amp;L EQUITIES, LLC</associatedBDName>
                <associatedBDCRDNumber>113794</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>1175 PEACHTREE STREET, NE, SUITE 2200</street1>
                    <city>ATLANTA</city>
                    <stateOrCountry>GA</stateOrCountry>
                    <stateOrCountryDescription>GEORGIA</stateOrCountryDescription>
                    <zipCode>30361</zipCode>
                </recipientAddress>
                <statesOfSolicitationList>
                    <state>GA</state>
                    <description>GEORGIA</description>
                    <state>TX</state>
                    <description>TEXAS</description>
                    <state>FL</state>
                    <description>FLORIDA</description>
                </statesOfSolicitationList>
                <foreignSolicitation>false</foreignSolicitation>
            </recipient>
            <recipient>
                <recipientName>PATRICK J. WHELCHEL</recipientName>
                <recipientCRDNumber>5389090</recipientCRDNumber>
                <associatedBDName>H&amp;L EQUITIES, LLC</associatedBDName>
                <associatedBDCRDNumber>113794</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>1175 PEACHTREE STREET, NE, SUITE 2200</street1>
                    <city>ATLANTA</city>
                    <stateOrCountry>GA</stateOrCountry>
                    <stateOrCountryDescription>GEORGIA</stateOrCountryDescription>
                    <zipCode>30361</zipCode>
                </recipientAddress>
                <statesOfSolicitationList>
                    <state>GA</state>
                    <description>GEORGIA</description>
                </statesOfSolicitationList>
                <foreignSolicitation>false</foreignSolicitation>
            </recipient>
            <recipient>
                <recipientName>LORI MAYFIELD</recipientName>
                <recipientCRDNumber>4899271</recipientCRDNumber>
                <associatedBDName>H&amp;L EQUITIES, LLC</associatedBDName>
                <associatedBDCRDNumber>113794</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>1175 PEACHTREE STREET, NE, SUITE 2200</street1>
                    <city>ATLANTA</city>
                    <stateOrCountry>GA</stateOrCountry>
                    <stateOrCountryDescription>GEORGIA</stateOrCountryDescription>
                    <zipCode>30361</zipCode>
                </recipientAddress>
                <statesOfSolicitationList>
                    <state>GA</state>
                    <description>GEORGIA</description>
                </statesOfSolicitationList>
                <foreignSolicitation>false</foreignSolicitation>
            </recipient>
        </salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>55000</totalOfferingAmount>
            <totalAmountSold>55000</totalAmountSold>
            <totalRemaining>0</totalRemaining>
            <clarificationOfResponse></clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>110</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>2750</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
            <clarificationOfResponse>THE SALES COMMISSION PAYABLE TO H&amp;L EQUITIES, LLC IS 5% OF THE TOTAL CAPITAL RAISED.</clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
                <isEstimate>true</isEstimate>
            </grossProceedsUsed>
            <clarificationOfResponse></clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>2401 PENN AVENUE REIT, LLC</issuerName>
                <signatureName>/S/ NIKKI SPENCE</signatureName>
                <nameOfSigner>NIKKI SPENCE</nameOfSigner>
                <signatureTitle>ASSISTANT FINANCIAL CONTROLLER</signatureTitle>
                <signatureDate>2015-01-27</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
