Exhibit 23.1

CONSENT OFINDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We consent to the incorporation by reference in the Registration Statement Nos. 333-246346, 333-237577, 333-227974, 333-224800, 333-219104 and 333-219096 on Form S-3 and Registration Statement Nos. 333-237225, 333-229320, 333-222582, 333-221788, 333-218164, and 333-216897 on Form S-8 of our report dated March 12,2021, relating to the financial statements of Pulse Biosciences, Inc.appearing in this Annual Report on Form 10-K for the year ended December 31, 2020.

/s/DELOITTE & TOUCHE LLP

San Jose, California

March 12, 2021