X0609 4/A 2026-05-11 2026-05-11 0001625101 PULSE BIOSCIENCES, INC. PLSE 0001055919 DUGGAN ROBERT W false 601 BRICKELL KEY DRIVE SUITE 1080 MIAMI FL 33131 1 1 0 Common Stock 2026-05-11 4 P 0 660233 19.69 A 48370047 D Common Stock 630109 I Affiliated Company 1 Common Stock 450189 I Affiliated Company 2 The shares reported herein were acquired through the Company's at-the-market equity offering program established pursuant to that certain Equity Distribution Agreement, dated February 19, 2026, by and between the Company and TD Securities (USA) LLC, as sales agent. The shares were purchased during an open trading window under the Company's Insider Trading Policy and in accordance with applicable securities laws. The shares reported herein were acquired through the Company's at-the-market equity offering program at the prevailing market bid price or better at the time of sale. Includes approximately 700 shares inadvertently not reported in the Company's Schedule 14A filed on May 11, 2026. Shares are held by Genius 24C Inc., of which the Reporting Person is the sole shareholder. Shares are held by Blazon Corporation, of which the Reporting Person is the sole shareholder. /s/ Kenneth B. Stratton, as Attorney-in-Fact 2026-05-15