X0306 4 2020-10-01 0 0001623526 Stoke Therapeutics, Inc. STOK 0001780085 Nash Huw M. C/O STOKE THERAPEUTICS, INC. 45 WIGGINS AVENUE BEDFORD MA 01730 0 1 0 0 COO & CBO Common Stock 2020-10-01 4 M 0 5700 2.19 A 5700 D Common Stock 2020-10-01 4 S 0 3400 34.3288 D 2300 D Common Stock 2020-10-01 4 S 0 2300 34.862 D 0 D Employee Stock Option (Right to Buy) 2.19 2020-10-01 4 M 0 5700 0.00 D 2018-12-12 Common Stock 5700 155911 D The sales reported on this Form 4 were effected pursuant to a Rule 10b5-1 trading plan adopted by the reporting person. The reported price in Column 4 is a weighted average price. These shares were sold in multiple transactions at prices ranging from $33.65 to $34.61 per share, inclusive. The reporting person undertakes to provide to the issuer, any security holder of the issuer, or the staff of the Securities and Exchange Commission, upon request, full information regarding the number of shares sold at each separate price within the range set forth in this footnote. The reported price in Column 4 is a weighted average price. These shares were sold in multiple transactions at prices ranging from $34.635 to $35.00 per share, inclusive. The reporting person undertakes to provide to the issuer, any security holder of the issuer, or the staff of the Securities and Exchange Commission, upon request, full information regarding the number of shares sold at each separate price within the range set forth in this footnote. The option vested as to 1/48th of the total shares on November 22, 2018, and then 1/48th of the total shares vest monthly thereafter, subject to the reporting person's continued service to the issuer through each vesting date. /s/ Stephen Tulipano, Attorney-in-Fact 2021-10-05