X0306 4 2020-07-07 0 0001623526 Stoke Therapeutics, Inc. STOK 0001780085 Nash Huw M. C/O STOKE THERAPEUTICS, INC. 45 WIGGINS AVENUE BEDFORD MA 01730 0 1 0 0 COO & CBO Common Stock 2020-07-07 4 S 0 913 22.4062 D 0 D The sales reported on this Form 4 were effected pursuant to a Rule 10b5-1 trading plan adopted by the reporting person. The reported price in Column 4 is a weighted average price. These shares were sold in multiple transactions at prices ranging from $22.22 to $22.64 per share, inclusive. The reporting person undertakes to provide to the issuer, any security holder of the issuer, or the staff of the Securities and Exchange Commission, upon request, full information regarding the number of shares sold at each separate price within the range set forth in this footnote. Includes 913 shares of Common Stock acquired under the issuer's employee stock purchase plan on June 30, 2020. /s/ Stephen Tulipano, Attorney-in-Fact 2020-07-09