X0306 4 2022-07-18 0001621443 Zynerba Pharmaceuticals, Inc. ZYNE 0001219866 ANIDO ARMANDO C/O ZYNERBA PHARMACEUTICALS, INC. 80 W. LANCASTER AVENUE, SUITE 300 DEVON PA 19333 1 1 0 0 Chairman & CEO Common Stock 2022-07-18 4 A 0 43164 A 623587 D Common Stock 2022-07-19 4 S 0 11899 1.10 D 611688 D Common Stock 26595 I See footnote Common Stock 13297 I See footnote On July 18, 2022, the Compensation Committee of the Board of Directors of the Company determined that performance-vesting criteria were met with regard to 43,164 performance-based restricted stock awards granted in a prior year. This transaction represents shares required to be sold by the Reporting Person to satisfy tax obligations and certain equity plan administrator fees in connection with the vesting of shares of restricted stock. This sale was mandated by the equity plan administrator and does not represent a discretionary trade by the Reporting Person. TUA of Armando Anido and Nancy J. Anido Trusts are controlled by Armando Anido, who has voting and dispositive power with respect to shares held by the trusts. /s/ Albert P. Parker, Attorney-in-Fact 2022-07-20