X0306 4 2020-11-27 0 0001761612 BICYCLE THERAPEUTICS plc BCYC 0001618273 Kalowski Lee C/O BICYCLE THERAPEUTICS PLC B900, BABRAHAM RESEARCH CAMPUS CAMBRIDGE X0 CB22 3AT UNITED KINGDOM 0 1 0 0 President and CFO Ordinary Shares 2020-11-27 4 M 0 530 1.56 A 530 D Ordinary Shares 2020-11-27 4 S 0 530 20.00 D 0 D Stock Option (Right to Buy) 1.56 2020-11-27 4 M 0 530 0.00 D 2027-07-23 Ordinary Shares 530 112274 D Shares exercised and sold pursuant to a Rule 10b5-1 trading plan. This exercise price is in US Dollars and reflects the conversion of GBP to USD at an exchange rate of $1.33582 to GBP 1.00 as of November 27, 2020. 20% of the shares subject to the option vested on July 24, 2018, 60% of the shares subject to the option will vest each month thereafter in 36 equal monthly installments, and the remaining 20% of the shares subject to the option will vest on the earlier of (i) the fourth anniversary of the grant date and (ii) the date on which the Issuer's board of directors determines that the Issuer has satisfied certain performance vesting milestones, in all cases provided that the Reporting Person remains continuously employed with us through each applicable vesting date. /s/ Lee Kalowski 2020-11-30