<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0708</schemaVersion>

    <submissionType>D</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001614744</cik>
        <entityName>Kitov Pharmaceuticals Holdings Ltd.</entityName>
        <issuerAddress>
            <street1>ONE AZRIELI CENTER</street1>
            <street2>ROUND BUILDING</street2>
            <city>TEL AVIV</city>
            <stateOrCountry>L3</stateOrCountry>
            <stateOrCountryDescription>ISRAEL</stateOrCountryDescription>
            <zipCode>6701101</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>97239333121</issuerPhoneNumber>
        <jurisdictionOfInc>ISRAEL</jurisdictionOfInc>
        <issuerPreviousNameList>
            <previousName>Mainrom Line Logistics Ltd.</previousName>
        </issuerPreviousNameList>
        <edgarPreviousNameList>
            <value>None</value>
        </edgarPreviousNameList>
        <entityType>Other</entityType>
        <entityTypeOtherDesc>Limited company</entityTypeOtherDesc>
        <yearOfInc>
            <overFiveYears>true</overFiveYears>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Isaac</firstName>
                <lastName>Israel</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>One Azrieli Center</street1>
                <street2>Round Building</street2>
                <city>Tel Aviv</city>
                <stateOrCountry>L3</stateOrCountry>
                <stateOrCountryDescription>ISRAEL</stateOrCountryDescription>
                <zipCode>6701101</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Chief Executive Officer and Director of the Issuer</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>John Paul</firstName>
                <lastName>Waymack</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>One Azrieli Center</street1>
                <street2>Round Building</street2>
                <city>Tel Aviv</city>
                <stateOrCountry>L3</stateOrCountry>
                <stateOrCountryDescription>ISRAEL</stateOrCountryDescription>
                <zipCode>6701101</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Chief Medical Officer and Chairman of the Board of Directors</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Simcha</firstName>
                <lastName>Rock</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>One Azrieli Center</street1>
                <street2>Round Building</street2>
                <city>Tel Aviv</city>
                <stateOrCountry>L3</stateOrCountry>
                <stateOrCountryDescription>ISRAEL</stateOrCountryDescription>
                <zipCode>6701101</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Chief Financial Officer and Director</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Steven</firstName>
                <lastName>Steinberg</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>One Azrieli Center</street1>
                <street2>Round Building</street2>
                <city>Tel Aviv</city>
                <stateOrCountry>L3</stateOrCountry>
                <stateOrCountryDescription>ISRAEL</stateOrCountryDescription>
                <zipCode>6701101</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Director</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Ido</firstName>
                <lastName>Agmon</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>One Azrieli Center</street1>
                <street2>Round Building</street2>
                <city>Tel Aviv</city>
                <stateOrCountry>L3</stateOrCountry>
                <stateOrCountryDescription>ISRAEL</stateOrCountryDescription>
                <zipCode>6701101</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Director</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Arye</firstName>
                <lastName>Weber</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>One Azrieli Center</street1>
                <street2>Round Building</street2>
                <city>Tel Aviv</city>
                <stateOrCountry>L3</stateOrCountry>
                <stateOrCountryDescription>ISRAEL</stateOrCountryDescription>
                <zipCode>6701101</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Director</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Ran</firstName>
                <lastName>Tzror</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>One Azrieli Center</street1>
                <street2>Round Building</street2>
                <city>Tel Aviv</city>
                <stateOrCountry>L3</stateOrCountry>
                <stateOrCountryDescription>ISRAEL</stateOrCountryDescription>
                <zipCode>6701101</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Director</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Revital</firstName>
                <lastName>Stern-Raff</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>One Azrieli Center</street1>
                <street2>Round Building</street2>
                <city>Tel Aviv</city>
                <stateOrCountry>L3</stateOrCountry>
                <stateOrCountryDescription>ISRAEL</stateOrCountryDescription>
                <zipCode>6701101</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Director</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Gil</firstName>
                <lastName>Ben-Menachem</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>One Azrieli Center</street1>
                <street2>Round Building</street2>
                <city>Tel Aviv</city>
                <stateOrCountry>L3</stateOrCountry>
                <stateOrCountryDescription>ISRAEL</stateOrCountryDescription>
                <zipCode>6701101</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Vice President of Business Development</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Avraham</firstName>
                <lastName>Ben-Tzvi</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>One Azrieli Center</street1>
                <street2>Round Building</street2>
                <city>Tel Aviv</city>
                <stateOrCountry>L3</stateOrCountry>
                <stateOrCountryDescription>ISRAEL</stateOrCountryDescription>
                <zipCode>6701101</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Group Chief Legal Officer</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Hadas</firstName>
                <lastName>Reuveni</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>One Azrieli Center</street1>
                <street2>Round Building</street2>
                <city>Tel Aviv</city>
                <stateOrCountry>L3</stateOrCountry>
                <stateOrCountryDescription>ISRAEL</stateOrCountryDescription>
                <zipCode>6701101</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Founder and Chief Technology Officer of TyrNovo</relationshipClarification>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Pharmaceuticals</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>Decline to Disclose</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06b</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>false</isAmendment>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2017-07-11</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>false</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isOptionToAcquireType>true</isOptionToAcquireType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>0</minimumInvestmentAccepted>
        <salesCompensationList>
            <recipient>
                <recipientName>Rodman &amp; Renshaw, a unit of H.C. Wainwright &amp; Co., LLC</recipientName>
                <recipientCRDNumber>375</recipientCRDNumber>
                <associatedBDName>None</associatedBDName>
                <associatedBDCRDNumber>None</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>430 PARK AVENUE</street1>
                    <street2>4TH FLOOR</street2>
                    <city>NEW YORK</city>
                    <stateOrCountry>NY</stateOrCountry>
                    <stateOrCountryDescription>NEW YORK</stateOrCountryDescription>
                    <zipCode>10022</zipCode>
                </recipientAddress>
                <statesOfSolicitationList>
                    <state>CA</state>
                    <description>CALIFORNIA</description>
                    <state>IL</state>
                    <description>ILLINOIS</description>
                    <state>NJ</state>
                    <description>NEW JERSEY</description>
                    <state>NY</state>
                    <description>NEW YORK</description>
                </statesOfSolicitationList>
                <foreignSolicitation>false</foreignSolicitation>
            </recipient>
        </salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>1981005</totalOfferingAmount>
            <totalAmountSold>1981005</totalAmountSold>
            <totalRemaining>0</totalRemaining>
            <clarificationOfResponse>Represents the aggregate amount received from investors in connection with Issuer's registered offering of ADSs and concurrent private placement of warrants, respectively, which were issued jointly to investors for a single purchase price.</clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>14</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>138670</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
            <clarificationOfResponse>Represents 6% commission and 1% management fee of gross proceeds from investors for sale of ADSs and warrants in the registered offering and concurrent private placement. We will also pay expenses up to $75,000 and issued an unregistered warrant.</clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
            <clarificationOfResponse></clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>Kitov Pharmaceuticals Holdings Ltd.</issuerName>
                <signatureName>/s/ Simcha Rock</signatureName>
                <nameOfSigner>Simcha Rock</nameOfSigner>
                <signatureTitle>Chief Financial Officer and Director</signatureTitle>
                <signatureDate>2017-08-08</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
