SCHEDULE 13G/A 0001104659-23-100854 0001633120 XXXXXXXX LIVE 3 Ordinary Shares, nominal value Euro 0.04 per share 12/31/2024 0001612940 ProQR Therapeutics N.V. N71542109 Zernikedreef 9 Leiden P7 2333 CK Rule 13d-1(c) Van Herk Investments B.V. P7 11461995.00 0.00 11461995.00 0.00 11461995.00 N 10.9 CO Van Herk Investments THI B.V. P7 0.00 11461995.00 0.00 11461995.00 11461995.00 N 10.9 CO Van Herk Private Equity Investments B.V. P7 0.00 11461995.00 0.00 11461995.00 11461995.00 N 10.9 CO Stichting Administratiekantoor Penulata P7 0.00 11461995.00 0.00 11461995.00 11461995.00 N 10.9 OO Van Herk Management Services B.V. P7 0.00 11461995.00 0.00 11461995.00 11461995.00 N 10.9 CO Onroerend Goed Beheer- en Beleggingsmaatschappij A. van Herk B.V. P7 0.00 11461995.00 0.00 11461995.00 11461995.00 N 10.9 CO A. van Herk Holding B.V. P7 0.00 11461995.00 0.00 11461995.00 11461995.00 N 10.9 CO Stichting Administratiekantoor Abchrys P7 0.00 11461995.00 0.00 11461995.00 11461995.00 N 10.9 OO Adrianus van Herk P7 0.00 11461995.00 0.00 11461995.00 11461995.00 N 10.9 IN ProQR Therapeutics N.V. Zernikedreef 9, 2333 CK Leiden, the Netherlands This statement is being filed by (i) Van Herk Investments B.V., a private company with limited liability incorporated under the laws of the Netherlands ("VHI"), with respect to Ordinary Shares, nominal value Euro 0.04 per share (the "Ordinary Shares"), beneficially owned by it, (ii) Van Herk Investments THI B.V., a private company with limited liability incorporated under the laws of the Netherlands ("VHIT"), with respect to Ordinary Shares beneficially owned by VHI, (iii) Van Herk Private Equity Investments B.V., a private company with limited liability incorporated under the laws of the Netherlands ("VHPI"), with respect to Ordinary Shares beneficially owned by VHI and VHIT, (iv) Stichting Administratiekantoor Penulata, a foundation organized under the laws of the Netherlands ("Penulata"), with respect to Ordinary Shares beneficially owned by VHI, VHIT and VHPI, (v) Van Herk Management Services B.V., a private company with limited liability incorporated under the laws of the Netherlands ("VHMS"), with respect to Ordinary Shares beneficially owned by VHI, VHIT and VHPI, (vi) Onroerend Goed Beheer- en Beleggingsmaatschappij A. van Herk B.V., a private company with limited liability incorporated under the laws of the Netherlands ("OGBBA"), with respect to Ordinary Shares beneficially owned by VHI, VHIT, VHPI and VHMS, (vii) A. van Herk Holding B.V., a private company with limited liability incorporated under the laws of the Netherlands ("Holdings"), with respect to Ordinary Shares beneficially owned by VHI, VHIT, VHPI, VHMS and OGBBA, (viii) Stichting Administratiekantoor Abchrys, a foundation organized under the laws of the Netherlands ("Abchrys"), with respect to Ordinary Shares beneficially owned by VHI, VHIT, VHPI, VHMS, OGBBA and Holdings, and (ix) Adrianus van Herk ("Mr. van Herk") with respect to Ordinary Shares beneficially owned by VHI, VHIT, VHPI, VHMS, OGBBA, Holdings, Penulata and Abchrys. Mr. van Herk is (i) an investor, (ii) the holder of all of the depositary receipts issued by Penulata and Abchrys, (iii) the sole board member of Penulata and Abchrys, and (iv) the sole managing director of VHMS, OGBBA and Holdings. Penulata holds substantially all of the issued and outstanding shares of VHPI. VHPI is the sole shareholder of VHIT. VHIT is the sole shareholder of VHI. VHI is principally engaged in making investments. Abchrys holds substantially all of the issued and outstanding shares of Holdings. Holdings is the sole shareholder of OGBBA. OGBBA is the sole shareholder of VHMS and is principally engaged in making investments. VHMS is the sole managing director of VHI, VHIT and VHPI. Each of Mr. van Herk, VHIT, VHPI, Penulata, VHMS, OGBBA, Holdings and Abchrys disclaims beneficial ownership of the securities covered by this statement. The principal business address of each of Mr. van Herk, VHI, VHIT, VHPI, Penulata, VHMS, OGBBA, Holdings and Abchrys is: Lichtenauerlaan 30 3062 ME Rotterdam The Netherlands Each of VHI, VHIT, VHPI, VHMS, OGBBA and Holdings is a private company with limited liability incorporated under the laws of the Netherlands. Penulata and Abchrys are Netherlands foundations. Mr. van Herk is a citizen of the Netherlands. Y 11,461,995 shares of Ordinary Shares 10.9% (The percentages used in this statement are calculated based upon the 105,212,527 Ordinary Shares issued and outstanding as reported by the Issuer to the Reporting Persons as being the number of issued and outstanding Ordinary Shares as of December 31, 2024.) VHI 11,461,995 VHIT 0 VHPI 0 Penulata 0 VHMS 0 OGBBA 0 Holdings 0 Abchrys 0 Mr. van Herk 0 VHI 0 VHIT 11,461,995 VHPI 11,461,995 Penulata 11,461,995 VHMS 11,461,995 OGBBA 11,461,995 Holdings 11,461,995 Abchrys 11,461,995 Mr. van Herk 11,461,995 VHI 11,461,995 VHIT 0 VHPI 0 Penulata 0 VHMS 0 OGBBA 0 Holdings 0 Abchrys 0 Mr. van Herk 0 VHI 0 VHIT 11,461,995 VHPI 11,461,995 Penulata 11,461,995 VHMS 11,461,995 OGBBA 11,461,995 Holdings 11,461,995 Abchrys 11,461,995 Mr. van Herk 11,461,995 VHI has the sole power to vote or direct the vote, and to dispose or to direct the disposition of, the Ordinary Shares beneficially owned by it. VHIT shares the power to vote or direct the vote of, and to dispose or direct the disposition of, the Ordinary Shares beneficially owned by VHI by virtue of its direct equity interest in VHI. VHPI shares the power to vote or direct the vote of, and to dispose or direct the disposition of, the Ordinary Shares beneficially owned by VHI by virtue of its direct equity interest in VHIT. Penulata shares the power to vote or direct the vote of, and to dispose or direct the disposition of, the Ordinary Shares beneficially owned by VHI by virtue of its direct equity interest in VHPI. VHMS shares the power to vote or direct the vote of, and to dispose or direct the disposition of, the Ordinary Shares beneficially owned by VHI by virtue of it being the sole managing director of VHI, VHIT and VHPI. OGBBA shares the power to vote or direct the vote of, and to dispose or direct the disposition of, the Ordinary Shares beneficially owned by VHI by virtue of its direct equity interest in VHMS. Holdings shares the power to vote or direct the vote of, and to dispose or direct the disposition of, the Ordinary Shares beneficially owned by VHI by virtue of its direct equity interest in OGBBA. Abchrys shares the power to vote or direct the vote of, and to dispose or direct the disposition of, the Ordinary Shares beneficially owned by VHI by virtue of its direct equity interest in Holdings. Mr. van Herk shares the power to vote or direct the vote of, and to dispose or direct the disposition of, the Ordinary Shares beneficially owned by VHI by virtue of his holding of all of the depositary receipts issued by Penulata and Abchrys, his being the sole board member of Penulata and Abchrys, and his being the sole managing director of VHMS, OGBBA and Holdings. Y Y Y Y Y N By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under ?? 240.14a-11. 99.1 Joint Filing Agreement, dated February 14, 2025, by and among the Reporting Persons, as required by Rule 13d-1(k)(1) under the Securities Exchange Act of 1934, as amended. 99.2 Power of Attorney, dated as of September 16, 2019, by and among the Reporting Persons, incorporated by reference herein by reference to Exhibit 99.2 to Amendment No. 3 to Schedule 13G filed by the Reporting Persons on September 17, 2019 with respect to Zealand Pharma A/S (File No. 001-38178). Van Herk Investments B.V. /s/ Erik G.A. Esveld 02/14/2025 Van Herk Investments THI B.V. /s/ Erik G.A. Esveld 02/14/2025 Van Herk Private Equity Investments B.V. /s/ Erik G.A. Esveld 02/14/2025 Stichting Administratiekantoor Penulata /s/ Erik G.A. Esveld 02/14/2025 Van Herk Management Services B.V. /s/ Erik G.A. Esveld 02/14/2025 Onroerend Goed Beheer- en Beleggingsmaatschappij A. van Herk B.V. /s/ Erik G.A. Esveld 02/14/2025 A. van Herk Holding B.V. /s/ Erik G.A. Esveld 02/14/2025 Stichting Administratiekantoor Abchrys /s/ Erik G.A. Esveld 02/14/2025 Adrianus van Herk /s/ Erik G.A. Esveld 02/14/2025 * Pursuant to a Power of Attorney, dated as of September 16, 2019, by and among the Reporting Persons.