144 0001965622 XXXXXXXX LIVE 0001612042 Ascendis Pharma A/S 001-36815 TUBORG BOULEVARD 12 HELLERUP P7 1105 BP 45 70 22 22 44 JENSEN MICHAEL WOLFF Officer EXECUTUVE VP CHIEF LEGAL OFFICER ORDINARY SHARES CITIGROUP GLOBAL MARKETS

390 GREENWICH ST NEW YORK NY 10013

18349 4442109.00 61977408 03/02/2026 NASDAQ ORDINARY SHARES 03/02/2026 RESTRICTED STOCK VESTING ASCENDIS PHARMA A/S N 18349 03/02/2026 COMPENSATION N MICHAEL WOLFF JENSEN

TUBORG BOULEVARD 12 HELLERUP G7 DK2900

ORDINARY SHARES 12/04/2025 10000 2081018.69 SECTION 3 D BASED ON INDICATIVE MARKET PRICE 242.09.SHARES BEING SOLD FOR THE VESTING AND SETTLEMENT OF EQUITY AWARDS. UNDER SECURITIES TO BE SOLD DATE ACQUIRED ARE VARIOUS DATES.THE SALES ARE BEING PERFORMED OVER MULTIPLE DAYS. THESE ARE NON DISCRETIONARY TRANSACTIONS. THESE ARE BEING PERFORMED UNDER AN ESTABLISHED 10B51 PLAN 03/02/2026 09/07/2023 CONFORMED SIGNATURE ON FILE