144 0001965612 XXXXXXXX LIVE 0001612042 Ascendis Pharma A/S 001-36815 TUBORG BOULEVARD 12 HELLERUP P7 1105 BP 45 70 22 22 44 JENSEN FLEMMING STEEN Officer EXECUTIVE VP HEAD OF PRODUCT SUPPLY AND QUALITY ORDINARY SHARES CITIGROUP GLOBAL MARKETS

390 GREENWICH ST NEW YORK NY 10013

10800 2614572.00 61977408 03/02/2026 NASDAQ ORDINARY SHARES 03/02/2026 RESTRICTED STOCK VESTING ASCENDIS PHARMA A/S N 18349 03/02/2026 COMPENSATION N JENSEN FLEMMING STEEN

TUBORG BOULEVARD 12 HELLERUP G7 DK2900

ORDINARY SHARES 12/02/2025 10000 2058454.18 UNDER SECTION 3D INDICATIVE MARKET PRICE IS 242.09. SHARES BEING SOLD FOR THE VESTING AND SETTLEMENT OF EQUITY AWARDS. UNDER SECURITIES TO BE SOLD DATE ACQUIRED ARE VARIOUS DATES.THE INTENDED NUMBER OF SHARES IS AN APPRROXIMATION DUE TO MARKET CONDITIONS. THE SALES WILL TAKE PLACE OVER MULTIPLE DAYS. THESE ARE NON DISCRETIONARY TRANSACTIONS. THESE ARE BEING PERFORMED UNDER AN ESTABLISHED 10B51 PLAN 03/02/2026 09/07/2023 CONFORMED SIGNATURE ON FILE