144 0001180765 XXXXXXXX LIVE 0001612042 Ascendis Pharma A/S 001-36815 TUBORG BOULEVARD 12 HELLERUP G7 DK2900 45 70 22 22 44 MIKKELSEN JAN MOLLER Officer PRESIDENT CEO AMERICAN DEPOSITORY SHARES CITIGROUP GLOBAL MARKETS

390 GREENWICH ST NEW YORK NY 10013

86860 18413630.85 61695211 01/13/2026 NASDAQ AMERICAN DEPOSITORY SHARES 01/13/2026 RESTRICTED STOCK AND PERFORMANCE STOCK UNIT PROGRAM ASCENDIS PHARMA A/S N 86860 01/13/2026 COMPENSATION N MIKKELSEN JAN MOLLER

TUBORG BOULEVARD 12 HELLERUP G7 DK2900

AMERICAN DEPOSITORY SHARES 12/02/2025 100000 20675000.00 UNDER SECURITIES TO BE SOLD THEY WERE GRANTED IN TRANCHES RANGING FROM 2023 THRU AND INLCUDING 2025 THE SHARES REPORTED ON THIS FORM 144 WILL BE SOLD PURSUANT TO A RULE 10B51 TRADING PLAN 01/13/2026 09/02/2025 CONFORMED SIGNATURE ON FILE