144 0002048323 XXXXXXXX LIVE 0001612042 Ascendis Pharma A/S 001-36815 TUBORG BOULEVARD 12 HELLERUP G7 DK-2900 45 70 22 22 44 Shu Aimee Officer EXECUTIVE VICE PRESIDENT CHIEF MEDICAL OFFICER ORDINARY SHARES CITIGROUP GLOBAL MARKETS

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4204 700412.45 57707439 05/06/2025 NASDAQ ORDINARY SHARES 05/06/2025 ASCENDIS SHARE PLAN ASCENDIS PHARMA A/S N 4204 05/06/2025 COMPENSATION Y UNDER SECURITIES TO BE SOLD DATE ACQUIRED AND DATE PAYMENT VARIOUS YEARS. SHARES WERE SOLD FROM THE ASCENDIS SHARE PLAN AGGREGATE FMV USED INDICATIVE MARKET PRICE 166.606196 05/07/2025 CONFORMED SIGNATURE ON FILE