X0306 4 2022-03-14 0 0001603978 AquaBounty Technologies, Inc. AQB 0001762123 Wulf Sylvia C/O AQUABOUNTY TECHNOLOGIES, INC. 2 MILL & MAIN PLACE, SUITE 395 MAYNARD MA 01754 1 1 0 0 CEO & Director Common Stock 164088 D Common Stock 36170 D Common Stock 9196 D Common Stock 2022-03-14 4 A 0 14035 0.00 A 14035 D Options to Purchase Common Stock 2.22 2020-04-30 2029-04-30 Common Stock 150000 150000 D Options to Purchase Common Stock 1.88 2023-03-12 2030-03-12 Common Stock 31649 31649 D Options to Purchase Common Stock 6.72 2024-03-10 2031-03-10 Common Stock 8047 8047 D Options to Purchase Common Stock 1.52 2022-03-14 4 A 0 36842 0.00 A 2025-03-14 2032-03-14 Common Stock 36842 36842 D Award of restricted stock granted on 3/12/2020 pursuant to the AquaBounty Technologies, Inc. 2016 Equity Incentive Plan and are fully vested. Award of restricted stock granted on 3/10/2021 pursuant to the AquaBounty Technologies, Inc. 2016 Equity Incentive Plan that vests immediately as to 1/3 of the grant, with an additional 1/3 vesting 1 year after the grant, and the final 1/3 vesting 2 years after the date of grant. Award of restricted stock granted on 3/14/2022 pursuant to the AquaBounty Technologies, Inc. 2016 Equity Incentive Plan that vests immediately as to 1/3 of the grant, with an additional 1/3 vesting 1 year after the grant, and the final 1/3 vesting 2 years after the date of grant. Options to purchase common stock were granted on 4/30/2019 pursuant to the AquaBounty Technologies, Inc. 2016 Equity Incentive Plan and are fully vested. Options to purchase common stock were granted on 3/12/2020 pursuant to the AquaBounty Technologies, Inc. 2016 Equity Incentive Plan and fully vest three years after the date of grant. Options to purchase common stock were granted on 3/10/2021 pursuant to the AquaBounty Technologies, Inc. 2016 Equity Incentive Plan and fully vest three years after the date of grant. Options to purchase common stock were granted on 3/14/2022 pursuant to the AquaBounty Technologies, Inc. 2016 Equity Incentive Plan and fully vest three years after the date of grant. Director, Chief Executive Officer, and President /s/ David A. Frank, Name: David A. Frank, attorney-in-fact 2022-03-16