<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0508</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2023-08-05</periodOfReport>

    <issuer>
        <issuerCik>0000842023</issuerCik>
        <issuerName>BIO-TECHNE Corp</issuerName>
        <issuerTradingSymbol>TECH</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001603594</rptOwnerCik>
            <rptOwnerName>Hippel James</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>614 MCKINLEY PLACE NE</rptOwnerStreet1>
            <rptOwnerStreet2></rptOwnerStreet2>
            <rptOwnerCity>MINNEAPOLIS</rptOwnerCity>
            <rptOwnerState>MN</rptOwnerState>
            <rptOwnerZipCode>55413</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>1</isOfficer>
            <isTenPercentOwner>0</isTenPercentOwner>
            <isOther>0</isOther>
            <officerTitle>CFO</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

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                <value>94.52</value>
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                <footnoteId id="F7"/>
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            <expirationDate>
                <value>2029-08-15</value>
            </expirationDate>
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                <underlyingSecurityTitle>
                    <value>Common Stock</value>
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    <footnotes>
        <footnote id="F1">Fully exercisable.</footnote>
        <footnote id="F2">Each restricted stock unit represents a contingent right to receive one share of Bio-Techne Corporation common stock.</footnote>
        <footnote id="F3">On 8/5/2023, the Board of Directors certified vesting of 12,876 performance RSUs.</footnote>
        <footnote id="F4">Options to purchase 57,440 shares are vested.  Options to purchase 19,148 shares vest on 8/5/2024.</footnote>
        <footnote id="F5">Vests in full or in part on 8/6/2024 if certain performance goals are achieved (or such later date as performance is certified by the Administrator).</footnote>
        <footnote id="F6">Options to purchase 20,268 shares are vested.  Options to purchase 10,136 shares vest on 8/6/2024 and options to purchase 10,134 shares vest on 8/6/2025.</footnote>
        <footnote id="F7">Vests in full or in part on 8/15/2025 if certain performance goals are achieved (or such later date as performance is certified by the Administrator).</footnote>
        <footnote id="F8">Options to purchase 11,304 shares vest on each of 8/15/2023, 8/15/2025 and 8/15/2026 and options to purchase 11,308 shares vest on 8/15/2024.</footnote>
    </footnotes>

    <remarks>On November 28, 2022, the Company completed a four-for-one stock split. All common stock amounts and exercise prices in the filing relating to securities acquired prior to November 28, 2022 have been adjusted to reflect the four-for-one stock split.</remarks>

    <ownerSignature>
        <signatureName>/s/ Andrew Nick, Attorney-in-Fact for James Hippel pursuant to Power of Attorney previously filed</signatureName>
        <signatureDate>2023-08-08</signatureDate>
    </ownerSignature>
</ownershipDocument>
