X0306 4 2020-05-19 0 0001781983 Aprea Therapeutics, Inc. APRE 0001589315 5AM Ventures IV, L.P. 501 2ND STREET, SUITE 350 SAN FRANCISCO CA 94107 0 0 1 0 0001593361 5AM Co-Investors IV, L.P. 501 2ND STREET, SUITE 350 SAN FRANCISCO CA 94107 0 0 1 0 0001589314 5AM Partners IV, LLC 501 2ND STREET, SUITE 350 SAN FRANCISCO CA 94107 0 0 1 0 0001240357 DIEKMAN JOHN D 501 2ND STREET, SUITE 350 SAN FRANCISCO CA 94107 0 0 1 0 0001598549 Schwab Andrew J. 501 2ND STREET, SUITE 350 SAN FRANCISCO CA 94107 0 0 1 0 0001753000 5AM Opportunities I, L.P. 501 2ND STREET, SUITE 350 SAN FRANCISCO CA 94107 0 0 1 0 0001790778 5AM Opportunities I (GP), LLC 501 2ND STREET, SUITE 350 SAN FRANCISCO CA 94107 0 0 1 0 0001664281 PARMAR KUSH 501 2ND STREET, SUITE 350 SAN FRANCISCO CA 94107 0 0 1 0 Common Stock 2020-05-19 4 S 0 244800 35.15 D 2173748 I See footnotes Common Stock 2020-05-19 4 S 0 10200 35.15 D 90572 I See footnotes Common Stock 333333 I See footnotes Shares are held directly by 5AM Ventures IV, L.P. 5AM Partners IV, LLC is the sole general partner of each of 5AM Ventures IV, L.P. and 5AM Co-Investors IV, L.P. (collectively, the "5AM IV Funds"). Dr. John D. Diekman, Andrew J. Schwab and Dr. Scott M. Rocklage are managing members of 5AM Partners IV, LLC and may be deemed to have shared voting and investment power over the shares beneficially owned by the 5AM IV Funds. Each of 5AM Partners IV, LLC, Dr. Diekman, Mr. Schwab and Dr. Rocklage disclaims beneficial ownership of the shares of Common Stock and Preferred Stock held directly by the 5AM IV Funds, except to the extent of its or his pecuniary interest therein. Shares are held directly by 5AM Co-Investors IV, L.P. Shares are held directly by 5AM Opportunities I, L.P. ("Opportunities"), which is under common control with the 5AM IV Funds. The 5AM IV Funds and Opportunities expressly disclaim status as a "group" for purposes of this Form 4. 5AM Opportunities I (GP), LLC ("Opportunities GP") is the sole general partner of Opportunities. Andrew J. Schwab and Dr. Kush Parmar are managing members of Opportunities GP and may be deemed to have shared voting and investment power over the shares beneficially owned by Opportunities. Each of Opportunities GP, Mr. Schwab and Dr. Parmar disclaims beneficial ownership of the shares of Common Stock held directly by Opportunities, except to the extent of its or his pecuniary interest therein. /s/ Andrew J. Schwab, Managing Member of 5AM Partners IV, LLC the General Partner of 5AM Ventures IV, L.P. 2020-05-21 /s/ Scott M. Rocklage, Managing Member of 5AM Partners IV, LLC, the General Parnter of 5AM Co-Investors IV, L.P. 2020-05-21 /s/ Scott M. Rocklage, Managing Member of 5AM Partners IV, LLC 2020-05-21 /s/ John D. Diekman 2020-05-21 /s/ Andrew J. Schwab 2020-05-21 /s/ Kush Parmar, Managing Member of 5AM Opportunities I (GP), LLC, the General Partner of 5AM Opportunities I, L.P. 2020-05-21 /s/ Kush Parmar, Managing Member of 5AM Opportunities I (GP), LLC 2020-05-21 /s/ Kush Parmar 2020-05-21