X0306 4 2020-10-05 1 0001781983 Aprea Therapeutics, Inc. APRE 0001589315 5AM Ventures IV, L.P. 501 2ND STREET, SUITE 350 SAN FRANCISCO CA 94107 0 0 0 1 Former 10% holder 0001593361 5AM Co-Investors IV, L.P. 501 2ND STREET, SUITE 350 SAN FRANCISCO CA 94107 0 0 0 1 Former 10% holder 0001589314 5AM Partners IV, LLC 501 2ND STREET, SUITE 350 SAN FRANCISCO CA 94107 0 0 0 1 Former 10% holder 0001753000 5AM Opportunities I, L.P. 501 2ND STREET, SUITE 350 SAN FRANCISCO CA 94107 0 0 0 1 Former 10% holder 0001790778 5AM Opportunities I (GP), LLC 501 2ND STREET, SUITE 350 SAN FRANCISCO CA 94107 0 0 0 1 Former 10% holder 0001240357 DIEKMAN JOHN D 501 2ND STREET, SUITE 350 SAN FRANCISCO CA 94107 0 0 0 1 Former 10% holder 0001598549 Schwab Andrew J. 501 2ND STREET, SUITE 350 SAN FRANCISCO CA 94107 0 0 0 1 Former 10% holder 0001664281 PARMAR KUSH 501 2ND STREET, SUITE 350 SAN FRANCISCO CA 94107 0 0 0 1 Former 10% holder 0001219014 ROCKLAGE SCOTT M 501 2ND STREET, SUITE 350 SAN FRANCISCO CA 94107 0 0 0 1 Former 10% holder Common Stock 2020-10-05 4 S 0 175000 25.30 D 138497 I See footnotes Common Stock 1823192 I See footnotes Common Stock 75965 I See footnotes Shares are held directly by 5AM Opportunities I, L.P. ("Opportunities"), which is under common control with the 5AM IV Funds. The 5AM IV Funds and Opportunities expressly disclaim status as a "group" for purposes of this Form 4. 5AM Opportunities I (GP), LLC ("Opportunities GP") is the sole general partner of Opportunities. Andrew J. Schwab and Dr. Kush Parmar are managing members of Opportunities GP and may be deemed to have shared voting and investment power over the shares beneficially owned by Opportunities. Each of Opportunities GP, Mr. Schwab and Dr. Parmar disclaims beneficial ownership of the shares of Common Stock held directly by Opportunities, except to the extent of its or his pecuniary interest therein. Shares are held directly by 5AM Ventures IV, L.P. 5AM Partners IV, LLC is the sole general partner of each of 5AM Ventures IV, L.P. and 5AM Co-Investors IV, L.P. (collectively, the "5AM IV Funds"). Dr. John D. Diekman, Andrew J. Schwab and Dr. Scott M. Rocklage are managing members of 5AM Partners IV, LLC and may be deemed to have shared voting and investment power over the shares beneficially owned by the 5AM IV Funds. Each of 5AM Partners IV, LLC, Dr. Diekman, Dr. Scott M. Rocklage and Mr. Schwab disclaims beneficial ownership of the shares of Common Stock held directly by the 5AM IV Funds, except to the extent of its or his pecuniary interest therein. Shares are held directly by 5AM Co-Investors IV, L.P. 5AM VENTURES IV, L.P. By: 5AM Partners IV, LLC, its General Partner By: /s/ Scott M. Rocklage Managing Member 2020-10-07 5AM CO-INVESTORS IV, L.P. By: 5AM Partners IV, LLC, its General Partner By: /s/ Scott M. Rocklage Managing Member 2020-10-07 5AM PARTNERS IV, LLC By: /s/ Scott M. Rocklage Managing Member 2020-10-07 5AM OPPORTUNITIES I, L.P. By: 5AM Opportunities I (GP), LLC, its General Partner By: /s/ Kush Parmar Managing Member 2020-10-07 5AM OPPORTUNITIES I (GP), LLC By: /s/ Kush Parmar Managing Member 2020-10-07 /s/ John D. Diekman 2020-10-07 /s/ Andrew J. Schwab 2020-10-07 /s/ Kush Parmar 2020-10-07 /s/ Scott M. Rocklage 2020-10-07