<?xml version="1.0" encoding="UTF-8"?><own:edgarSubmission xmlns:com="http://www.sec.gov/edgar/common" xmlns:own="http://www.sec.gov/edgar/ownership">
  <!-- Generated by Workiva -->
  <own:headerData>
    <own:submissionType>144</own:submissionType>
    <own:filerInfo>
      <own:filer>
        <own:filerCredentials>
          <own:cik>0001490448</own:cik>
          <own:ccc>XXXXXXXX</own:ccc>
        </own:filerCredentials>
      </own:filer>
      <own:liveTestFlag>LIVE</own:liveTestFlag>



    </own:filerInfo>
  </own:headerData>
  <own:formData>
    <own:issuerInfo>
      <own:issuerCik>0001595527</own:issuerCik>
      <own:issuerName>American Strategic Investment Co.</own:issuerName>
      <own:secFileNumber>001-39448</own:secFileNumber>
      <own:issuerAddress>
        <com:street1>222 Bellevue Avenue</com:street1>
        <com:city>Newport</com:city>
        <com:stateOrCountry>RI</com:stateOrCountry>
        <com:zipCode>02840</com:zipCode>
      </own:issuerAddress>
      <own:issuerContactPhone>2124156500</own:issuerContactPhone>
      <own:nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>EDWARD M WEIL JR</own:nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
      <own:relationshipsToIssuer>
        <own:relationshipToIssuer>Director</own:relationshipToIssuer>
      </own:relationshipsToIssuer>
    </own:issuerInfo>
    <own:securitiesInformation>
      <own:securitiesClassTitle>Common</own:securitiesClassTitle>
      <own:brokerOrMarketmakerDetails>
        <own:name>Merrill Lynch</own:name>
        <own:address>
          <com:street1>75 Rockefeller Plaza</com:street1>
          <com:city>New York</com:city>
          <com:stateOrCountry>NY</com:stateOrCountry>
          <com:zipCode>10019</com:zipCode>
        </own:address>
      </own:brokerOrMarketmakerDetails>
      <own:noOfUnitsSold>7500</own:noOfUnitsSold>
      <own:aggregateMarketValue>67500</own:aggregateMarketValue>
      <own:noOfUnitsOutstanding>2919885</own:noOfUnitsOutstanding>
      <own:approxSaleDate>08/11/2026</own:approxSaleDate>
      <own:securitiesExchangeName>NYSE</own:securitiesExchangeName>
    </own:securitiesInformation>
    <own:securitiesToBeSold>
      <own:securitiesClassTitle>Common</own:securitiesClassTitle>
      <own:acquiredDate>03/26/2021</own:acquiredDate>
      <own:natureOfAcquisitionTransaction>Purchase</own:natureOfAcquisitionTransaction>
      <own:nameOfPersonfromWhomAcquired>NA</own:nameOfPersonfromWhomAcquired>
      <own:isGiftTransaction>N</own:isGiftTransaction>
      <own:amountOfSecuritiesAcquired>19</own:amountOfSecuritiesAcquired>
      <own:paymentDate>03/26/2021</own:paymentDate>
      <own:natureOfPayment>Cash</own:natureOfPayment>
    </own:securitiesToBeSold>
    <own:securitiesToBeSold>
      <own:securitiesClassTitle>Common</own:securitiesClassTitle>
      <own:acquiredDate>03/29/2021</own:acquiredDate>
      <own:natureOfAcquisitionTransaction>Purchase</own:natureOfAcquisitionTransaction>
      <own:nameOfPersonfromWhomAcquired>NA</own:nameOfPersonfromWhomAcquired>
      <own:isGiftTransaction>N</own:isGiftTransaction>
      <own:amountOfSecuritiesAcquired>19</own:amountOfSecuritiesAcquired>
      <own:paymentDate>03/29/2021</own:paymentDate>
      <own:natureOfPayment>Cash</own:natureOfPayment>
    </own:securitiesToBeSold>
    <own:securitiesToBeSold>
      <own:securitiesClassTitle>Common</own:securitiesClassTitle>
      <own:acquiredDate>03/30/2021</own:acquiredDate>
      <own:natureOfAcquisitionTransaction>Purchase</own:natureOfAcquisitionTransaction>
      <own:nameOfPersonfromWhomAcquired>NA</own:nameOfPersonfromWhomAcquired>
      <own:isGiftTransaction>N</own:isGiftTransaction>
      <own:amountOfSecuritiesAcquired>19</own:amountOfSecuritiesAcquired>
      <own:paymentDate>03/30/2021</own:paymentDate>
      <own:natureOfPayment>Cash</own:natureOfPayment>
    </own:securitiesToBeSold>
    <own:securitiesToBeSold>
      <own:securitiesClassTitle>Common</own:securitiesClassTitle>
      <own:acquiredDate>02/27/2023</own:acquiredDate>
      <own:natureOfAcquisitionTransaction>Purchase</own:natureOfAcquisitionTransaction>
      <own:nameOfPersonfromWhomAcquired>NA</own:nameOfPersonfromWhomAcquired>
      <own:isGiftTransaction>N</own:isGiftTransaction>
      <own:amountOfSecuritiesAcquired>11</own:amountOfSecuritiesAcquired>
      <own:paymentDate>02/27/2023</own:paymentDate>
      <own:natureOfPayment>Cash</own:natureOfPayment>
    </own:securitiesToBeSold>
    <own:securitiesToBeSold>
      <own:securitiesClassTitle>Common</own:securitiesClassTitle>
      <own:acquiredDate>03/02/2023</own:acquiredDate>
      <own:natureOfAcquisitionTransaction>Purchase</own:natureOfAcquisitionTransaction>
      <own:nameOfPersonfromWhomAcquired>NA</own:nameOfPersonfromWhomAcquired>
      <own:isGiftTransaction>N</own:isGiftTransaction>
      <own:amountOfSecuritiesAcquired>40</own:amountOfSecuritiesAcquired>
      <own:paymentDate>03/02/2023</own:paymentDate>
      <own:natureOfPayment>Purchase</own:natureOfPayment>
    </own:securitiesToBeSold>
    <own:securitiesToBeSold>
      <own:securitiesClassTitle>Common</own:securitiesClassTitle>
      <own:acquiredDate>07/09/2026</own:acquiredDate>
      <own:natureOfAcquisitionTransaction>Compensation</own:natureOfAcquisitionTransaction>
      <own:nameOfPersonfromWhomAcquired>na</own:nameOfPersonfromWhomAcquired>
      <own:isGiftTransaction>N</own:isGiftTransaction>
      <own:amountOfSecuritiesAcquired>300000</own:amountOfSecuritiesAcquired>
      <own:paymentDate>07/09/2026</own:paymentDate>
      <own:natureOfPayment>Compensation</own:natureOfPayment>
    </own:securitiesToBeSold>
    <own:nothingToReportFlagOnSecuritiesSoldInPast3Months>Y</own:nothingToReportFlagOnSecuritiesSoldInPast3Months>
    <own:remarks>Fractional shares were rounded to nearest whole number</own:remarks>
    <own:noticeSignature>
      <own:noticeDate>08/13/2026</own:noticeDate>
      <own:signature>Edward Weil</own:signature>
    </own:noticeSignature>
  </own:formData>
</own:edgarSubmission>
