<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3</documentType>

    <periodOfReport>2015-10-21</periodOfReport>

    <noSecuritiesOwned>0</noSecuritiesOwned>

    <issuer>
        <issuerCik>0001592288</issuerCik>
        <issuerName>Dimension Therapeutics, Inc.</issuerName>
        <issuerTradingSymbol>DMTX</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001653056</rptOwnerCik>
            <rptOwnerName>Wadsworth Samuel C.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O DIMENSION THERAPEUTICS, INC.</rptOwnerStreet1>
            <rptOwnerStreet2>840 MEMORIAL DRIVE, 4TH FLOOR</rptOwnerStreet2>
            <rptOwnerCity>CAMBRIDGE</rptOwnerCity>
            <rptOwnerState>MA</rptOwnerState>
            <rptOwnerZipCode>02139</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>1</isOfficer>
            <isTenPercentOwner>0</isTenPercentOwner>
            <isOther>0</isOther>
            <officerTitle>Chief Scientific Officer</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Common Stock</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>242247</value>
                    <footnoteId id="F1"/>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Common Stock</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>35788</value>
                    <footnoteId id="F2"/>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">These shares were acquired pursuant to a restricted stock grant under the Company's 2013 Stock Plan. These shares vest over four (48) months beginning on October 30, 2013, at a rate of twenty-five percent (25%) after 12 months and in thirty-six (36) equal monthly installments thereafter.</footnote>
        <footnote id="F2">These shares were acquired pursuant to a restricted stock grant under the Company's 2013 Stock Plan. These shares vest over four (48) months beginning on November 1, 2013, at a rate of twenty-five percent (25%) after 12 months and in thirty-six (36) equal monthly installments thereafter.</footnote>
    </footnotes>

    <remarks>Exhibit 24.1: Limited Power of Attorney</remarks>

    <ownerSignature>
        <signatureName>/s/ Mary Thistle, as Attorney-in-Fact for Samuel C. Wadsworth</signatureName>
        <signatureDate>2015-10-21</signatureDate>
    </ownerSignature>
</ownershipDocument>
