Exhibit 21.1

Twist Bioscience Corporation Subsidiaries

Twist Bioscience Corporation has the following subsidiaries:

1. Twist Bioscience Worldwide, a Cayman Islands exempted company.

2. Genome Compiler Corporation, a Delaware corporation, which itself owns Twist Bioscience Israel Ltd. (formerly “Genome Compiler Israel Ltd.”), an Israeli company.

3. Twist Bio Computing, LLC, a Delaware limited liability company.

4. Twist Pharmaceutical Solutions, LLC, a Delaware limited liability company.

5. Twist Bioscience Singapore PTE. LTD., a Singapore company, which itself owns Twist Bioscience (China) LLC, a Chinese limited liability company.