SCHEDULE 13G/A 0001104659-21-002915 0001697748 XXXXXXXX LIVE 6 Common Stock 02/28/2025 0001581280 Twist Bioscience Corp 90184D100 681 Gateway Blvd. South San Francisco CA 94080 Rule 13d-1(b) ARK Investment Management LLC DE 6077112.00 107575.00 6382166.00 0.00 6382166.00 N 10.70 IA Twist Bioscience Corp 681 Gateway Blvd, South San Francisco, CA, 94080 ARK Investment Management LLC 200 Central Avenue, St. Petersburg, FL 33701 Delaware N IA 6,382,166 10.70 6,077,112 107,575 6,382,166 0 Y N To the knowledge of the Reporting Person, no other person has the right to receive or the power to direct the receipt of dividends from, or the proceeds from the sale of, a number of the shares which represents more than five percent of the number of shares outstanding class of the shares. Y Y Y N By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were acquired and are held in the ordinary course of business and were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under ?? 240.14a-11. ARK Investment Management LLC /s/ Kellen Carter 03/07/2025