<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0707</schemaVersion>

    <submissionType>D/A</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001574437</cik>
        <entityName>Guepardo Investment Fund</entityName>
        <issuerAddress>
            <street1>AVE. BRIGADEIRO FARIA LIMA, 3311</street1>
            <street2>SUITE 41</street2>
            <city>SAO PAULO</city>
            <stateOrCountry>D5</stateOrCountry>
            <stateOrCountryDescription>BRAZIL</stateOrCountryDescription>
            <zipCode>04538-133</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>55 (11) 3103 9200</issuerPhoneNumber>
        <jurisdictionOfInc>CAYMAN ISLANDS</jurisdictionOfInc>
        <issuerPreviousNameList>
            <value>None</value>
        </issuerPreviousNameList>
        <edgarPreviousNameList>
            <value>None</value>
        </edgarPreviousNameList>
        <entityType>Corporation</entityType>
        <yearOfInc>
            <withinFiveYears>true</withinFiveYears>
            <value>2011</value>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Octavio</firstName>
                <middleName>Ferreira</middleName>
                <lastName>de Magalhaes</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>Av. Brigadeiro Faria Lima, 3311</street1>
                <street2>Suite 41</street2>
                <city>Sao Paulo</city>
                <stateOrCountry>D5</stateOrCountry>
                <stateOrCountryDescription>BRAZIL</stateOrCountryDescription>
                <zipCode>04538-133</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Ricardo</firstName>
                <middleName>Retz</middleName>
                <lastName>de Carvalho</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>Av. Brigadeiro Faria Lima, 3311</street1>
                <street2>Suite 41</street2>
                <city>Sao Paulo</city>
                <stateOrCountry>D5</stateOrCountry>
                <stateOrCountryDescription>BRAZIL</stateOrCountryDescription>
                <zipCode>04538-133</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Roberto</firstName>
                <middleName>De Magalhaes</middleName>
                <lastName>Esteves</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>Av. Brigadeiro Faria Lima, 3311</street1>
                <street2>Suite 41</street2>
                <city>Sao Paulo</city>
                <stateOrCountry>D5</stateOrCountry>
                <stateOrCountryDescription>BRAZIL</stateOrCountryDescription>
                <zipCode>04538-133</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>N/A</firstName>
                <lastName>Guepardo Investimentos Ltd.</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>Av. Brigadeiro Faria Lima, 3311</street1>
                <street2>Suite 41</street2>
                <city>Sao Paulo</city>
                <stateOrCountry>D5</stateOrCountry>
                <stateOrCountryDescription>BRAZIL</stateOrCountryDescription>
                <zipCode>04538-133</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Promoter</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Investment Manager of the Issuer</relationshipClarification>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Pooled Investment Fund</industryGroupType>
            <investmentFundInfo>
                <investmentFundType>Hedge Fund</investmentFundType>
                <is40Act>false</is40Act>
            </investmentFundInfo>
        </industryGroup>
        <issuerSize>
            <aggregateNetAssetValueRange>Decline to Disclose</aggregateNetAssetValueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06b</item>
            <item>3C</item>
            <item>3C.7</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>true</isAmendment>
                <previousAccessionNumber>0001507943-13-000077</previousAccessionNumber>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2011-09-30</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>true</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isPooledInvestmentFundType>true</isPooledInvestmentFundType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
            <clarificationOfResponse></clarificationOfResponse>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>1000000</minimumInvestmentAccepted>
        <salesCompensationList>
            <recipient>
                <recipientName>SILVER LEAF PARTNERS, LLC</recipientName>
                <recipientCRDNumber>126694</recipientCRDNumber>
                <associatedBDName>None</associatedBDName>
                <associatedBDCRDNumber>None</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>420 Lexington Ave</street1>
                    <street2>Suite 2225</street2>
                    <city>New York</city>
                    <stateOrCountry>NY</stateOrCountry>
                    <stateOrCountryDescription>NEW YORK</stateOrCountryDescription>
                    <zipCode>10170-2200</zipCode>
                </recipientAddress>
                <statesOfSolicitationList>
                    <value>All States</value>
                </statesOfSolicitationList>
                <foreignSolicitation>false</foreignSolicitation>
            </recipient>
            <recipient>
                <recipientName>SPOONHILL ASSET MANAGEMENT INC.</recipientName>
                <recipientCRDNumber>122377</recipientCRDNumber>
                <associatedBDName>None</associatedBDName>
                <associatedBDCRDNumber>None</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>63 SPOONHILL AVENUE</street1>
                    <city>MARLBOROUGH</city>
                    <stateOrCountry>MA</stateOrCountry>
                    <stateOrCountryDescription>MASSACHUSETTS</stateOrCountryDescription>
                    <zipCode>01752</zipCode>
                </recipientAddress>
                <statesOfSolicitationList>
                    <state>GA</state>
                    <description>GEORGIA</description>
                    <state>MA</state>
                    <description>MASSACHUSETTS</description>
                    <state>NY</state>
                    <description>NEW YORK</description>
                    <state>TX</state>
                    <description>TEXAS</description>
                </statesOfSolicitationList>
                <foreignSolicitation>false</foreignSolicitation>
            </recipient>
            <recipient>
                <recipientName>UNIPAR DEVELOPMENT CORPORATION</recipientName>
                <recipientCRDNumber>None</recipientCRDNumber>
                <associatedBDName>None</associatedBDName>
                <associatedBDCRDNumber>None</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>Calle 54 Este</street1>
                    <street2>Mossfon Building WTC, 2nd Floor</street2>
                    <city>Panama City</city>
                    <stateOrCountry>R1</stateOrCountry>
                    <stateOrCountryDescription>PANAMA</stateOrCountryDescription>
                    <zipCode>0832-0886</zipCode>
                </recipientAddress>
                <statesOfSolicitationList></statesOfSolicitationList>
                <foreignSolicitation>true</foreignSolicitation>
            </recipient>
            <recipient>
                <recipientName>Zellinvest SA</recipientName>
                <recipientCRDNumber>None</recipientCRDNumber>
                <associatedBDName>None</associatedBDName>
                <associatedBDCRDNumber>None</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>Rue Albert-Gos, 3</street1>
                    <city>Geneve</city>
                    <stateOrCountry>V8</stateOrCountry>
                    <stateOrCountryDescription>SWITZERLAND</stateOrCountryDescription>
                    <zipCode>CH-1206</zipCode>
                </recipientAddress>
                <statesOfSolicitationList></statesOfSolicitationList>
                <foreignSolicitation>true</foreignSolicitation>
            </recipient>
            <recipient>
                <recipientName>Viclan Group</recipientName>
                <recipientCRDNumber>None</recipientCRDNumber>
                <associatedBDName>None</associatedBDName>
                <associatedBDCRDNumber>None</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>Botanica Tower Ste, 2009</street1>
                    <street2>P.O. Box 66709</street2>
                    <city>Dubai</city>
                    <stateOrCountry>C0</stateOrCountry>
                    <stateOrCountryDescription>UNITED ARAB EMIRATES</stateOrCountryDescription>
                    <zipCode>337-1500</zipCode>
                </recipientAddress>
                <statesOfSolicitationList></statesOfSolicitationList>
                <foreignSolicitation>true</foreignSolicitation>
            </recipient>
        </salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>Indefinite</totalOfferingAmount>
            <totalAmountSold>1264300</totalAmountSold>
            <totalRemaining>Indefinite</totalRemaining>
            <clarificationOfResponse></clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>1</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>0</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
            <clarificationOfResponse>To the best of our knowledge no commission or finders' fee has yet been paid in relation to US investors.</clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
            <clarificationOfResponse></clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>Guepardo Investment Fund</issuerName>
                <signatureName>Roberto de Magalhaes Esteves</signatureName>
                <nameOfSigner>Roberto de Magalhaes Esteves</nameOfSigner>
                <signatureTitle>Director</signatureTitle>
                <signatureDate>2014-02-12</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
