X0206 3/A 2024-02-01 2024-02-01 0 0001572616 FRACTYL HEALTH, INC. GUTS 0001667672 Rajagopalan Harith 3 VAN DE GRAAFF DRIVE, SUITE 200 BURLINGTON MA 01803 1 1 0 0 Chief Executive Officer Common Stock 364399 D Common Stock 292171 I By 2021 Irrevocable Trust Common Stock 289375 I By 2016 Irrevocable Trust Common Stock 18639 I By 2021 Family Trust Series A Preferred Stock Common Stock 6523 D Series A Preferred Stock Common Stock 2795 I By 2016 Irrevocable Trust Stock Option 1.70 2024-11-11 Common Stock 190968 D Stock Option 1.70 2025-02-10 Common Stock 123484 D Stock Option 2.67 2025-12-17 Common Stock 345078 D Stock Option 2.67 2026-06-27 Common Stock 179868 D Stock Option 3.35 2028-03-14 Common Stock 422473 D Stock Option 3.89 2030-03-26 Common Stock 475021 D Stock Option 6.98 2031-06-24 Common Stock 262937 D Stock Option 8.59 2032-09-07 Common Stock 16309 D Stock Option 8.18 2033-03-16 Common Stock 32618 D Stock Option 11.21 2033-11-10 Common Stock 17031 D Restricted Stock Units Common Stock 216924 D Each share of the Issuer's preferred stock is convertible into shares of the Issuer's common stock at the holder's election in accordance with the terms of such preferred stock and has no expiration date. The preferred stock will automatically convert into common stock upon the closing of the Issuer's initial public offering. The stock option is fully vested and currently exercisable. The stock option vests in 48 monthly installments beginning on April 26, 2020. The stock option vests in 48 monthly installments beginning on July 24, 2021. The stock option vests in 48 monthly installments beginning on October 7, 2022. The stock option vests in 48 monthly installments beginning on April 16, 2023. The stock option vests in 48 monthly installments beginning on December 10, 2023. Each restricted stock unit represents a contingent right to receive one share of the Issuer's common stock. The restricted stock units will vest on November 10, 2024 and have no expiration date. Exhibit 24 - Power of Attorney. This amendment to the Reporting Person's Form 3, originally filed on February 1, 2024, is filed solely to reflect a transfer from the Reporting Person to the Harith Rajagopalan 2021 Irrevocable Trust effective prior to February 1, 2024. /s/ Lisa A. Davidson, Attorney-in-fact 2024-05-14