<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0609</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2026-04-30</periodOfReport>

    <notSubjectToSection16>1</notSubjectToSection16>

    <issuer>
        <issuerCik>0001901637</issuerCik>
        <issuerName>USCB FINANCIAL HOLDINGS, INC.</issuerName>
        <issuerTradingSymbol>USCB</issuerTradingSymbol>
        <issuerForeignTradingSymbol></issuerForeignTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0000949197</rptOwnerCik>
            <rptOwnerName>WYCOFF W KIRK</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerNonUSAddressFlag>false</rptOwnerNonUSAddressFlag>
            <rptOwnerStreet1>FOUR RADNOR CORPORATE CENTER</rptOwnerStreet1>
            <rptOwnerStreet2>100 MATSONFORD ROAD, SUITE 210</rptOwnerStreet2>
            <rptOwnerCity>RADNOR</rptOwnerCity>
            <rptOwnerState>PA</rptOwnerState>
            <rptOwnerZipCode>19087</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isTenPercentOwner>1</isTenPercentOwner>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0002061817</rptOwnerCik>
            <rptOwnerName>Patriot Financial Partners GP II, L.P.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerNonUSAddressFlag>false</rptOwnerNonUSAddressFlag>
            <rptOwnerStreet1>FOUR RADNOR CORPORATE CENTER</rptOwnerStreet1>
            <rptOwnerStreet2>100 MATSONFORD ROAD, SUITE 210</rptOwnerStreet2>
            <rptOwnerCity>RADNOR</rptOwnerCity>
            <rptOwnerState>PA</rptOwnerState>
            <rptOwnerZipCode>19087</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isTenPercentOwner>1</isTenPercentOwner>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001583223</rptOwnerCik>
            <rptOwnerName>Patriot Financial Partners II, L.P.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerNonUSAddressFlag>false</rptOwnerNonUSAddressFlag>
            <rptOwnerStreet1>FOUR RADNOR CORPORATE CENTER</rptOwnerStreet1>
            <rptOwnerStreet2>100 MATSONFORD ROAD, SUITE 210</rptOwnerStreet2>
            <rptOwnerCity>RADNOR</rptOwnerCity>
            <rptOwnerState>PA</rptOwnerState>
            <rptOwnerZipCode>19087</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isTenPercentOwner>1</isTenPercentOwner>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001586224</rptOwnerCik>
            <rptOwnerName>Patriot Financial Partners Parallel II, L.P.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerNonUSAddressFlag>false</rptOwnerNonUSAddressFlag>
            <rptOwnerStreet1>FOUR RADNOR CORPORATE CENTER</rptOwnerStreet1>
            <rptOwnerStreet2>100 MATSONFORD ROAD, SUITE 210</rptOwnerStreet2>
            <rptOwnerCity>RADNOR</rptOwnerCity>
            <rptOwnerState>PA</rptOwnerState>
            <rptOwnerZipCode>19087</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isTenPercentOwner>1</isTenPercentOwner>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0002061814</rptOwnerCik>
            <rptOwnerName>Patriot Financial Partners GP II, LLC</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerNonUSAddressFlag>false</rptOwnerNonUSAddressFlag>
            <rptOwnerStreet1>FOUR RADNOR CORPORATE CENTER</rptOwnerStreet1>
            <rptOwnerStreet2>100 MATSONFORD ROAD, SUITE 210</rptOwnerStreet2>
            <rptOwnerCity>RADNOR</rptOwnerCity>
            <rptOwnerState>PA</rptOwnerState>
            <rptOwnerZipCode>19087</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isTenPercentOwner>1</isTenPercentOwner>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001561111</rptOwnerCik>
            <rptOwnerName>Deutsch James F.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerNonUSAddressFlag>false</rptOwnerNonUSAddressFlag>
            <rptOwnerStreet1>FOUR RADNOR CORPORATE CENTER</rptOwnerStreet1>
            <rptOwnerStreet2>100 MATSONFORD ROAD, SUITE 210</rptOwnerStreet2>
            <rptOwnerCity>RADNOR</rptOwnerCity>
            <rptOwnerState>PA</rptOwnerState>
            <rptOwnerZipCode>19087</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isTenPercentOwner>1</isTenPercentOwner>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001203115</rptOwnerCik>
            <rptOwnerName>LUBERT IRA M</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerNonUSAddressFlag>false</rptOwnerNonUSAddressFlag>
            <rptOwnerStreet1>FMC TOWER AT CIRA CENTRE SOUTH</rptOwnerStreet1>
            <rptOwnerStreet2>2929 WALNUT STREET, SUITE 1550</rptOwnerStreet2>
            <rptOwnerCity>PHILADELPHIA</rptOwnerCity>
            <rptOwnerState>PA</rptOwnerState>
            <rptOwnerZipCode>19104</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isTenPercentOwner>1</isTenPercentOwner>
            <otherText></otherText>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001222379</rptOwnerCik>
            <rptOwnerName>LYNCH JAMES J</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerNonUSAddressFlag>false</rptOwnerNonUSAddressFlag>
            <rptOwnerStreet1>FOUR RADNOR CORPORATE CENTER</rptOwnerStreet1>
            <rptOwnerStreet2>100 MATSONFORD ROAD, SUITE 210</rptOwnerStreet2>
            <rptOwnerCity>RADNOR</rptOwnerCity>
            <rptOwnerState>PA</rptOwnerState>
            <rptOwnerZipCode>19087</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isTenPercentOwner>1</isTenPercentOwner>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0002079600</rptOwnerCik>
            <rptOwnerName>Patriot Financial Manager, L.P.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerNonUSAddressFlag>false</rptOwnerNonUSAddressFlag>
            <rptOwnerStreet1>FOUR RADNOR CORPORATE CENTER</rptOwnerStreet1>
            <rptOwnerStreet2>100 MATSONFORD ROAD, SUITE 210</rptOwnerStreet2>
            <rptOwnerCity>RADNOR</rptOwnerCity>
            <rptOwnerState>PA</rptOwnerState>
            <rptOwnerZipCode>19087</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isTenPercentOwner>1</isTenPercentOwner>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0002079601</rptOwnerCik>
            <rptOwnerName>Patriot Financial Manager, LLC</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerNonUSAddressFlag>false</rptOwnerNonUSAddressFlag>
            <rptOwnerStreet1>FOUR RADNOR CORPORATE CENTER</rptOwnerStreet1>
            <rptOwnerStreet2>100 MATSONFORD ROAD, SUITE 210</rptOwnerStreet2>
            <rptOwnerCity>RADNOR</rptOwnerCity>
            <rptOwnerState>PA</rptOwnerState>
            <rptOwnerZipCode>19087</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isTenPercentOwner>1</isTenPercentOwner>
        </reportingOwnerRelationship>
    </reportingOwner>

    <aff10b5One>0</aff10b5One>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Class A Voting Common Stock</value>
            </securityTitle>
            <transactionDate>
                <value>2026-04-30</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>S</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>37152</value>
                </transactionShares>
                <transactionPricePerShare>
                    <value>18.24</value>
                    <footnoteId id="F1"/>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>D</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>1826634</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                    <footnoteId id="F3"/>
                    <footnoteId id="F4"/>
                    <footnoteId id="F5"/>
                    <footnoteId id="F6"/>
                    <footnoteId id="F7"/>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Class A Voting Common Stock</value>
            </securityTitle>
            <transactionDate>
                <value>2026-05-01</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>S</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>12848</value>
                </transactionShares>
                <transactionPricePerShare>
                    <value>18.28</value>
                    <footnoteId id="F2"/>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>D</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>1813786</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                    <footnoteId id="F3"/>
                    <footnoteId id="F4"/>
                    <footnoteId id="F5"/>
                    <footnoteId id="F6"/>
                    <footnoteId id="F7"/>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
    </nonDerivativeTable>

    <derivativeTable>
        <derivativeHolding>
            <securityTitle>
                <value>Option to Purchase Class A Voting Common Stock</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>11.3500</value>
            </conversionOrExercisePrice>
            <exerciseDate>
                <value>2019-09-23</value>
            </exerciseDate>
            <expirationDate>
                <footnoteId id="F8"/>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Class A Common Stock</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>4000</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>4000</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                    <footnoteId id="F8"/>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeHolding>
    </derivativeTable>

    <footnotes>
        <footnote id="F1">The price reported in Column 4 is a weighted average price. These shares were sold in multiple transactions at prices ranging from $18.20 to $18.57, inclusive. The reporting persons undertake to provide to USCB, any security holder of USCB, or the staff of the Securities and Exchange Commission, upon request, full information regarding the number of shares sold at each separate price within the ranges set forth herein.</footnote>
        <footnote id="F2">The price reported in Column 4 is a weighted average price. These shares were sold in multiple transactions at prices ranging from $18.25 to $18.50, inclusive. The reporting persons undertake to provide to USCB, any security holder of USCB, or the staff of the Securities and Exchange Commission, upon request, full information regarding the number of shares sold at each separate price within the ranges set forth herein.</footnote>
        <footnote id="F3">This Form 4 is filed jointly by Patriot Financial Partners GP II, LLC (&quot;Patriot LLC&quot;), Patriot Financial Partners GP II, LP. (&quot;Patriot GP&quot;), Patriot Financial Partners II, LP. (&quot;Patriot Fund II&quot;), Patriot Financial Partners Parallel II, LP. (&quot;Patriot Parallel Fund II,&quot; together with Patriot Fund II, the &quot;Funds&quot;), Patriot Financial Manager, LLC (&quot;Manager LLC&quot;) and Patriot Financial Manager, L.P. (&quot;Manager LP&quot;), W. Kirk Wycoff, James J. Lynch, Ira M. Lubert and James F. Deutsch.</footnote>
        <footnote id="F4">Patriot GP is a general partner of each of the Funds and Patriot LLC is a general partner of Patriot GP. In addition, each of W. Kirk Wycoff, Ira M. Lubert and James J. Lynch serve as general partners of the Funds and is a member of Patriot LLC. Manager LP, serves as investment manager to the Funds. Manager LLC serves as general partner of Manager LP and W. Kirk Wycoff, Ira M. Lubert and James J. Lynch are members of Manager LLC. James F. Deutsch is a member of the Patriot Funds Investment Committee.</footnote>
        <footnote id="F5">The securities owned by the Funds may be regarded as being beneficially owned by Patriot GP, Patriot LLC, Manager LLC, Manager LP, W. Kirk Wycoff, James J. Lynch, Ira M. Lubert and James F. Deutsch.</footnote>
        <footnote id="F6">After the sales, Manager LP holds 7,500 shares of common stock, Patriot Fund II holds 1,617,670 shares of common stock and Patriot Parallel Fund II holds 188,616 shares of common stock.</footnote>
        <footnote id="F7">This filing shall not be deemed an admission that the Reporting Persons are subject to Section 16 of the Securities Exchange Act of 1934, as amended (the &quot;Exchange Act&quot;), or, for purposes of Section 16 of the Exchange Act or otherwise (other than to the extent a Reporting Person directly holds the securities reported herein), and Messrs. Wycoff, Lynch, Lubert and Deutsch each disclaim beneficial ownership of the securities owned by the Funds or Manager LP, except to the extent of their respective pecuniary interest therein.</footnote>
        <footnote id="F8">The option was previously granted to Mr. Wycoff as a director of the Company. The option to purchase Class A Voting Common Stock remains exercisable until three months after the date Mr. Wycoff ceased to serve as a non-employee of the Issuer.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ Patriot Financial Partners II, L.P. By: W. Kirk Wycoff, a member of Patriot LLC, the general partner of Patriot GP, the general partner of Patriot Fund II</signatureName>
        <signatureDate>2026-05-01</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ Patriot Financial Partners GP II, L.P. By: W. Kirk Wycoff, a member of Patriot LLC, the general partner of Patriot GP, the general partner of Patriot Fund II</signatureName>
        <signatureDate>2026-05-01</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ Patriot Financial Partners Parallel II, L.P. By: W. Kirk Wycoff, a member of Patriot LLC, the general partner of Patriot GP, the general partner of Patriot Parallel Fund II</signatureName>
        <signatureDate>2026-05-01</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ Patriot Financial Partners GP II, LLC By: W. Kirk Wycoff, a member</signatureName>
        <signatureDate>2026-05-01</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ Patriot Financial Manager, L.P. By: W. Kirk Wycoff a member of Manager LLC, the general partner of Manager LP</signatureName>
        <signatureDate>2026-05-01</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ Patriot Financial Manager LLC By: W. Kirk Wycoff, a member</signatureName>
        <signatureDate>2026-05-01</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ W. Kirk Wycoff</signatureName>
        <signatureDate>2026-05-01</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ James F. Deutsch</signatureName>
        <signatureDate>2026-05-01</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ James J. Murphy by P.O.A. for Ira M. Lubert</signatureName>
        <signatureDate>2026-05-01</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ James J. Lynch</signatureName>
        <signatureDate>2026-05-01</signatureDate>
    </ownerSignature>
</ownershipDocument>
