<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0609</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2026-05-29</periodOfReport>

    <notSubjectToSection16>1</notSubjectToSection16>

    <issuer>
        <issuerCik>0001547459</issuerCik>
        <issuerName>Natural Grocers by Vitamin Cottage, Inc.</issuerName>
        <issuerTradingSymbol>NGVC</issuerTradingSymbol>
        <issuerForeignTradingSymbol></issuerForeignTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001554582</rptOwnerCik>
            <rptOwnerName>Isely Charity</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerNonUSAddressFlag>false</rptOwnerNonUSAddressFlag>
            <rptOwnerStreet1>C/O NATURAL GROCERS BY VITAMIN COTTAGE</rptOwnerStreet1>
            <rptOwnerStreet2>12612 WEST ALAMEDA PARKWAY</rptOwnerStreet2>
            <rptOwnerCity>LAKEWOOD</rptOwnerCity>
            <rptOwnerState>CO</rptOwnerState>
            <rptOwnerZipCode>80228</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isOther>1</isOther>
            <otherText>13D Group Member</otherText>
        </reportingOwnerRelationship>
    </reportingOwner>

    <aff10b5One>0</aff10b5One>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Common Stock</value>
            </securityTitle>
            <transactionDate>
                <value>2026-05-29</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>S</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>3000</value>
                </transactionShares>
                <transactionPricePerShare>
                    <value>29.04</value>
                    <footnoteId id="F1"/>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>D</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>10697</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">This transaction was executed in multiple trades at prices ranging from $28.70 to $29.45. The price reported above reflects the weighted average price, rounded to the nearest hundredth. The Reporting Person undertakes to provide, upon request, to the SEC staff, the Issuer or any security holder of the Issuer full information regarding the number of shares sold at each separate price within the ranges described.</footnote>
    </footnotes>

    <remarks>As of the date of this report, the reporting person is no longer subject to the voting provisions of the Stockholders Agreement and thus is no longer a member of the Schedule 13D group that beneficially owns more than 10% of the issuer's common stock.</remarks>

    <ownerSignature>
        <signatureName>/s/ Kemper Isely, by Power of Attorney</signatureName>
        <signatureDate>2026-06-02</signatureDate>
    </ownerSignature>
</ownershipDocument>
