<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3/A</documentType>

    <periodOfReport>2022-08-17</periodOfReport>

    <dateOfOriginalSubmission>2022-08-29</dateOfOriginalSubmission>

    <noSecuritiesOwned>0</noSecuritiesOwned>

    <issuer>
        <issuerCik>0001515816</issuerCik>
        <issuerName>Plymouth Industrial REIT, Inc.</issuerName>
        <issuerTradingSymbol>PLYM</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001944086</rptOwnerCik>
            <rptOwnerName>MIRELF VI REIT Investments IV, LLC</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>300 PARK AVENUE</rptOwnerStreet1>
            <rptOwnerStreet2>3RD FLOOR</rptOwnerStreet2>
            <rptOwnerCity>NEW YORK</rptOwnerCity>
            <rptOwnerState>NY</rptOwnerState>
            <rptOwnerZipCode>10022</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isTenPercentOwner>true</isTenPercentOwner>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001944105</rptOwnerCik>
            <rptOwnerName>MIRELF VI (U.S.), LP</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>300 PARK AVENUE</rptOwnerStreet1>
            <rptOwnerStreet2>3RD FLOOR</rptOwnerStreet2>
            <rptOwnerCity>NEW YORK</rptOwnerCity>
            <rptOwnerState>NY</rptOwnerState>
            <rptOwnerZipCode>10022</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isTenPercentOwner>true</isTenPercentOwner>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001944084</rptOwnerCik>
            <rptOwnerName>Madison International Holdings VI, LLC</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>300 PARK AVENUE</rptOwnerStreet1>
            <rptOwnerStreet2>3RD FLOOR</rptOwnerStreet2>
            <rptOwnerCity>NEW YORK</rptOwnerCity>
            <rptOwnerState>NY</rptOwnerState>
            <rptOwnerZipCode>10022</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isTenPercentOwner>true</isTenPercentOwner>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001640680</rptOwnerCik>
            <rptOwnerName>Madison International Realty Holdings, LLC</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>300 PARK AVENUE</rptOwnerStreet1>
            <rptOwnerStreet2>3RD FLOOR</rptOwnerStreet2>
            <rptOwnerCity>NEW YORK</rptOwnerCity>
            <rptOwnerState>NY</rptOwnerState>
            <rptOwnerZipCode>10022</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isTenPercentOwner>true</isTenPercentOwner>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001944063</rptOwnerCik>
            <rptOwnerName>Madison International Realty Partners, LP</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>300 PARK AVENUE</rptOwnerStreet1>
            <rptOwnerStreet2>3RD FLOOR</rptOwnerStreet2>
            <rptOwnerCity>NEW YORK</rptOwnerCity>
            <rptOwnerState>NY</rptOwnerState>
            <rptOwnerZipCode>10022</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isTenPercentOwner>true</isTenPercentOwner>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001944067</rptOwnerCik>
            <rptOwnerName>Madison International Realty Partners GP, LLC</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>300 PARK AVENUE</rptOwnerStreet1>
            <rptOwnerStreet2>3RD FLOOR</rptOwnerStreet2>
            <rptOwnerCity>NEW YORK</rptOwnerCity>
            <rptOwnerState>NY</rptOwnerState>
            <rptOwnerZipCode>10022</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isTenPercentOwner>true</isTenPercentOwner>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001552332</rptOwnerCik>
            <rptOwnerName>Dickerman Ronald</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>300 PARK AVENUE</rptOwnerStreet1>
            <rptOwnerStreet2>3RD FLOOR</rptOwnerStreet2>
            <rptOwnerCity>NEW YORK</rptOwnerCity>
            <rptOwnerState>NY</rptOwnerState>
            <rptOwnerZipCode>10022</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isTenPercentOwner>true</isTenPercentOwner>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001661989</rptOwnerCik>
            <rptOwnerName>MIRELF VI REIT</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>300 PARK AVE</rptOwnerStreet1>
            <rptOwnerStreet2>3RD FLOOR</rptOwnerStreet2>
            <rptOwnerCity>NEW YORK</rptOwnerCity>
            <rptOwnerState>NY</rptOwnerState>
            <rptOwnerZipCode>10022</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isTenPercentOwner>true</isTenPercentOwner>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001944803</rptOwnerCik>
            <rptOwnerName>Madison International Realty VI, LLC</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>300 PARK AVENUE</rptOwnerStreet1>
            <rptOwnerStreet2>3RD FLOOR</rptOwnerStreet2>
            <rptOwnerCity>NEW YORK</rptOwnerCity>
            <rptOwnerState>NY</rptOwnerState>
            <rptOwnerZipCode>10022</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isTenPercentOwner>true</isTenPercentOwner>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Common Stock, par value $0.01 per share</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>5037730</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>See Footnotes</value>
                    <footnoteId id="F1"/>
                    <footnoteId id="F2"/>
                    <footnoteId id="F3"/>
                    <footnoteId id="F4"/>
                </natureOfOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">This statement  is being filed by MIRELF VI REIT Investments IV, LLC (&quot;Investments IV&quot;), MIRELF VI REIT, MIRELF VI (U.S.), LP (&quot;MIRELF VI US&quot;), Madison International Holdings VI, LLC (&quot;Holdings&quot;), Madison International Realty VI, LLC (&quot;Realty VI&quot;), Madison International Realty Holdings, LLC (&quot;Realty Holdings&quot;), Madison International Realty Partners, LP (&quot;Realty Partners&quot;), Madison Realty Partners GP (&quot;Realty Partners GP&quot;) and Ronald M. Dickerman (&quot;Mr. Dickerman&quot; and, together with Investments IV, MIRELF VI REIT, MIRELF VI US, Holdings, Realty VI, Realty Holdings, Realty Partners and Realty Partners GP, the &quot;Reporting Persons&quot;).</footnote>
        <footnote id="F2">Held directly by Investments IV.  MIRELF VI REIT is the sole shareholder and managing member of Investments IV and may be deemed to have beneficial ownership over the shares of Common Stock held by Investments IV. MIRELF VI US is the sole shareholder and trustee of MIRELF VI REIT and may be deemed to have beneficial ownership over the shares of Common Stock held by Investments IV over which MIRELF VI REIT shares beneficial ownership. Holdings is the general partner of MIRELF VI US and may be deemed to have beneficial ownership over the shares of Common Stock held by Investments IV over which MIRELF VI US shares beneficial ownership. Realty VI is the asset manager of MIRELF VI US and may be deemed to have beneficial ownership over the shares of Common Stock held by Investments IV over which MIRELF VI US shares beneficial ownership.</footnote>
        <footnote id="F3">Realty Holdings is the sole member of Realty VI and may be deemed to have beneficial ownership over the shares of Common Stock held by Investments IV over which Realty VI shares beneficial ownership. Realty Partners is the managing member of Realty Holdings and may be deemed to have beneficial ownership over the shares of Common Stock held by Investments IV over which Realty Holdings shares beneficial ownership. Realty Partners GP is the general partner to Realty Partners and may be deemed to have beneficial ownership over the shares of Common Stock held by Investments IV over which Realty Partners shares beneficial ownership. Mr. Dickerman is the managing member of each of Holdings and Realty Partners GP and may be deemed to have beneficial ownership over the shares of Common Stock held by Investments IV over which Holdings and Realty Partners GP share beneficial ownership.</footnote>
        <footnote id="F4">Each of the Reporting Persons other than Investments IV disclaims beneficial ownership of all reported shares except to the extent of its pecuniary interest therein and the inclusion of the shares in this report shall not be deemed to be an admission of beneficial ownership of the reported shares for the purposes of Section 16 or otherwise.</footnote>
    </footnotes>

    <remarks>The Form 3 filed on August 29, 2022 is hereby amended and restated solely to add MIRELF VI REIT and Madison International Realty VI, LLC as reporting persons.</remarks>

    <ownerSignature>
        <signatureName>MIRELF VI REIT INVESTMENTS IV, LLC, By: MIRELF VI REIT, its sole and managing member, By: MIRELF VI (U.S.), LP, its sole shareholder and trustee, By: Madison International Holdings VI, LLC, its general partner, By: /s/ Ronald M. Dickerman, its managing me</signatureName>
        <signatureDate>2022-08-29</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>Madison International Holdings VI, LLC, By: Ronald M. Dickerman, as managing member</signatureName>
        <signatureDate>2022-08-29</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>Madison International Realty VI, LLC, By: Madison International Realty Holdings, LLC, its managing member, By: Madison International Realty Partners, LP, its managing member, By: Madison International Partners GP, LLC, its general partner, By: /s/ Ronald</signatureName>
        <signatureDate>2022-08-29</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>Madison International Realty Holdings, LLC, its managing member, By: Madison International Realty Partners, LP, its managing member, By: Madison International Partners GP, LLC, its general partner By: /s/ Ronald M. Dickerman, its managing member</signatureName>
        <signatureDate>2022-08-29</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>Madison International Realty Partners, LP, By: Madison International Partners GP, LLC, its general partner By: /s/ Ronald M. Dickerman, its managing member</signatureName>
        <signatureDate>2022-08-29</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>Madison International Partners GP, LLC, its general partner, By: /s/ Ronald M. Dickerman, its managing member</signatureName>
        <signatureDate>2022-08-29</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>Ronald M. Dickerman, By: /s/ Ronald M. Dickerman</signatureName>
        <signatureDate>2022-08-29</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>MIRELF VI REIT, By: MIRELF VI (U.S.), LP, its sole shareholder and trustee, By: Madison International Holdings VI, LLC, its general partner, By: /s/ Ronald M. Dickerman, its managing member</signatureName>
        <signatureDate>2022-08-29</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>MIRELF VI (U.S.), LP, its sole shareholder and trustee, By: Madison International Holdings VI, LLC, its general partner, By: /s/ Ronald M. Dickerman, its managing member</signatureName>
        <signatureDate>2022-08-29</signatureDate>
    </ownerSignature>
</ownershipDocument>
