<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3</documentType>

    <periodOfReport>2022-03-07</periodOfReport>

    <noSecuritiesOwned>0</noSecuritiesOwned>

    <issuer>
        <issuerCik>0001547459</issuerCik>
        <issuerName>Natural Grocers by Vitamin Cottage, Inc.</issuerName>
        <issuerTradingSymbol>NGVC</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001914587</rptOwnerCik>
            <rptOwnerName>Kemper &amp; Virginia Isely 2012 Irrevocable Family Trust</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O NATURAL GROCERS BY VITAMIN COTTAGE</rptOwnerStreet1>
            <rptOwnerStreet2>12612 WEST ALAMEDA PARKWAY</rptOwnerStreet2>
            <rptOwnerCity>LAKEWOOD</rptOwnerCity>
            <rptOwnerState>CO</rptOwnerState>
            <rptOwnerZipCode>80228</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isOther>1</isOther>
            <otherText>13D Group Member</otherText>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Common Stock</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>550849</value>
                    <footnoteId id="F1"/>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">On March 7, 2022, the Reporting Person executed a Joinder Agreement pursuant to which it agreed that all shares of Common Stock are subject to the provisions of a Stockholders Agreement that contains voting agreements. As a result, the Reporting Person is member of a Schedule 13D group that beneficially owns more than 10% of the Issuer's Common Stock.</footnote>
    </footnotes>

    <remarks>Exhibit Index: Exhibit 24 &amp;#8211; Power of Attorney</remarks>

    <ownerSignature>
        <signatureName>/s/ Kemper Isely, By Power of Attorney</signatureName>
        <signatureDate>2022-03-09</signatureDate>
    </ownerSignature>
</ownershipDocument>
