<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0508</schemaVersion>

    <documentType>4/A</documentType>

    <periodOfReport>2023-07-20</periodOfReport>

    <dateOfOriginalSubmission>2023-07-21</dateOfOriginalSubmission>

    <issuer>
        <issuerCik>0001540159</issuerCik>
        <issuerName>Edesa Biotech, Inc.</issuerName>
        <issuerTradingSymbol>EDSA</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001978257</rptOwnerCik>
            <rptOwnerName>Marshall Patrick</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O EDESA BIOTECH, INC.</rptOwnerStreet1>
            <rptOwnerStreet2>100 SPY COURT</rptOwnerStreet2>
            <rptOwnerCity>MARKHAM</rptOwnerCity>
            <rptOwnerState>A6</rptOwnerState>
            <rptOwnerZipCode>L3R 5H6</rptOwnerZipCode>
            <rptOwnerStateDescription>ONTARIO, CANADA</rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>true</isDirector>
        </reportingOwnerRelationship>
    </reportingOwner>

    <aff10b5One>false</aff10b5One>

    <derivativeTable>
        <derivativeTransaction>
            <securityTitle>
                <value>Share Option (right to buy)</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>0.8277</value>
            </conversionOrExercisePrice>
            <transactionDate>
                <value>2023-07-20</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>A</transactionCode>
                <equitySwapInvolved>false</equitySwapInvolved>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>20000</value>
                </transactionShares>
                <transactionPricePerShare>
                    <value>0</value>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>A</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <exerciseDate>
                <footnoteId id="F1"/>
            </exerciseDate>
            <expirationDate>
                <value>2033-07-20</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Shares</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>20000</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>20000</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </derivativeTransaction>
    </derivativeTable>

    <footnotes>
        <footnote id="F1">This option vests monthly in equal proportions over 12 months beginning on the date of grant.</footnote>
    </footnotes>

    <remarks>This amendment on Form 4/A is being filed to amend the Form 4 filed on July 21, 2023, solely to correct an administrative error in the expiration date of the Share Option previously reported in Column 6 of Table II.</remarks>

    <ownerSignature>
        <signatureName>/s/ Patrick Marshall</signatureName>
        <signatureDate>2023-07-24</signatureDate>
    </ownerSignature>
</ownershipDocument>
