<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3</documentType>

    <periodOfReport>2023-07-15</periodOfReport>

    <noSecuritiesOwned>1</noSecuritiesOwned>

    <issuer>
        <issuerCik>0001540159</issuerCik>
        <issuerName>Edesa Biotech, Inc.</issuerName>
        <issuerTradingSymbol>EDSA</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001983036</rptOwnerCik>
            <rptOwnerName>Lemieux Stephen</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O EDESA BIOTECH, INC.</rptOwnerStreet1>
            <rptOwnerStreet2>100 SPY COURT</rptOwnerStreet2>
            <rptOwnerCity>MARKHAM</rptOwnerCity>
            <rptOwnerState>A6</rptOwnerState>
            <rptOwnerZipCode>L3R 5H6</rptOwnerZipCode>
            <rptOwnerStateDescription>ONTARIO, CANADA</rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isOfficer>true</isOfficer>
            <officerTitle>Chief Financial Officer</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <ownerSignature>
        <signatureName>/s/ Stephen Lemieux</signatureName>
        <signatureDate>2023-07-17</signatureDate>
    </ownerSignature>
</ownershipDocument>
