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    <schemaVersion>X0704</schemaVersion>

    <submissionType>D</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001532707</cik>
        <entityName>AMPCI Macquarie Infrastructure Management No 1 Limited, AMPCI Macquarie Infrastructure Management No 2 Limited, &amp; DUET Investment Holdings Ltd</entityName>
        <issuerAddress>
            <street1>LEVEL 12, 1 MARTIN PLACE</street1>
            <city>SYDNEY</city>
            <stateOrCountry>C3</stateOrCountry>
            <stateOrCountryDescription>AUSTRALIA</stateOrCountryDescription>
            <zipCode>2000</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>(61)2-8232-4491</issuerPhoneNumber>
        <jurisdictionOfInc>AUSTRALIA</jurisdictionOfInc>
        <issuerPreviousNameList>
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        <entityType>Business Trust</entityType>
        <yearOfInc>
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    </primaryIssuer>

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                <firstName>John</firstName>
                <lastName>Roberts</lastName>
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                <street1>Level 12, 1 Martin Place</street1>
                <city>Sydney</city>
                <stateOrCountry>C3</stateOrCountry>
                <stateOrCountryDescription>AUSTRALIA</stateOrCountryDescription>
                <zipCode>2000</zipCode>
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                <firstName>Philip</firstName>
                <lastName>Garling</lastName>
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                <street1>Level 12, 1 Martin Place</street1>
                <city>Sydney</city>
                <stateOrCountry>C3</stateOrCountry>
                <stateOrCountryDescription>AUSTRALIA</stateOrCountryDescription>
                <zipCode>2000</zipCode>
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            <relatedPersonRelationshipList>
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            <relationshipClarification>Director of each issuer</relationshipClarification>
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        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Michael</firstName>
                <lastName>Lee</lastName>
            </relatedPersonName>
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                <street1>Level 12, 1 Martin Place</street1>
                <city>Sydney</city>
                <stateOrCountry>C3</stateOrCountry>
                <stateOrCountryDescription>AUSTRALIA</stateOrCountryDescription>
                <zipCode>2000</zipCode>
            </relatedPersonAddress>
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                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Director of AMPCI Macquarie Infrastructure Management No 1 Limited</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Doug</firstName>
                <lastName>Halley</lastName>
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                <street1>Level 12, 1 Martin Place</street1>
                <city>Sydney</city>
                <stateOrCountry>C3</stateOrCountry>
                <stateOrCountryDescription>AUSTRALIA</stateOrCountryDescription>
                <zipCode>2000</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
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            <relationshipClarification>Director of AMPCI Macquarie Infrastructure Management No 1 Limited and DUET Investment Holdings Limited</relationshipClarification>
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        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Emma</firstName>
                <lastName>Stein</lastName>
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                <street1>Level 12, 1 Martin Place</street1>
                <city>Sydney</city>
                <stateOrCountry>C3</stateOrCountry>
                <stateOrCountryDescription>AUSTRALIA</stateOrCountryDescription>
                <zipCode>2000</zipCode>
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                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Director of AMPCI Macquarie Infrastructure Management No 1 Limited and DUET Investment Holdings Limited</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Ron</firstName>
                <lastName>Finlay</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>Level 12, 1 Martin Place</street1>
                <city>Sydney</city>
                <stateOrCountry>C3</stateOrCountry>
                <stateOrCountryDescription>AUSTRALIA</stateOrCountryDescription>
                <zipCode>2000</zipCode>
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            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
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            <relationshipClarification>Director of AMPCI Macquarie Infrastructure Management No 2 Limited and DUET Investment Holdings Limited</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Eric</firstName>
                <lastName>Goodwin</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>Level 12, 1 Martin Place</street1>
                <city>Sydney</city>
                <stateOrCountry>C3</stateOrCountry>
                <stateOrCountryDescription>AUSTRALIA</stateOrCountryDescription>
                <zipCode>2000</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
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            <relationshipClarification>Director of AMPCI Macquarie Infrastructure Management No 2 Limited</relationshipClarification>
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        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Duncan</firstName>
                <lastName>Sutherland</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>Level 12, 1 Martin Place</street1>
                <city>Sydney</city>
                <stateOrCountry>C3</stateOrCountry>
                <stateOrCountryDescription>AUSTRALIA</stateOrCountryDescription>
                <zipCode>2000</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
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            <relationshipClarification>Director of AMPCI Macquarie Infrastructure Management No 2 Limited</relationshipClarification>
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        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>David</firstName>
                <lastName>Bartholomew</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>Level 12, 1 Martin Place</street1>
                <city>Sydney</city>
                <stateOrCountry>C3</stateOrCountry>
                <stateOrCountryDescription>AUSTRALIA</stateOrCountryDescription>
                <zipCode>2000</zipCode>
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            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification>Chief Executive Officer</relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Christine</firstName>
                <lastName>Williams</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>Level 12, 1 Martin Place</street1>
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                <stateOrCountry>C3</stateOrCountry>
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                <zipCode>2000</zipCode>
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            <relatedPersonRelationshipList>
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            <relationshipClarification>Company Secretary</relationshipClarification>
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        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Leanne</firstName>
                <lastName>Pickering</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>Level 12, 1 Martin Place</street1>
                <city>Sydney</city>
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                <stateOrCountryDescription>AUSTRALIA</stateOrCountryDescription>
                <zipCode>2000</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
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            <relationshipClarification>Company Secretary</relationshipClarification>
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    <offeringData>
        <industryGroup>
            <industryGroupType>Other</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>Decline to Disclose</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06</item>
            <item>3C</item>
            <item>3C.7</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>false</isAmendment>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2011-08-22</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>false</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isEquityType>true</isEquityType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>true</isBusinessCombinationTransaction>
            <clarificationOfResponse>DUET acquired an additional 20% interest in the Dampier Bunbury Pipeline and an additional 20.1% interest in Multinet, and selling its 25.9% interest in WA Gas Networks and the subordinated debt due from WA Gas Networks.</clarificationOfResponse>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>250000</minimumInvestmentAccepted>
        <salesCompensationList>
            <recipient>
                <recipientName>Macquarie Capital (USA) Inc.</recipientName>
                <recipientCRDNumber>36368</recipientCRDNumber>
                <associatedBDName>Macquarie Capital (USA) Inc.</associatedBDName>
                <associatedBDCRDNumber>36368</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>125 West 55th Street</street1>
                    <city>New York</city>
                    <stateOrCountry>NY</stateOrCountry>
                    <stateOrCountryDescription>NEW YORK</stateOrCountryDescription>
                    <zipCode>10019</zipCode>
                </recipientAddress>
                <statesOfSolicitationList>
                    <state>CA</state>
                    <description>CALIFORNIA</description>
                    <state>MA</state>
                    <description>MASSACHUSETTS</description>
                    <state>MN</state>
                    <description>MINNESOTA</description>
                    <state>NY</state>
                    <description>NEW YORK</description>
                    <state>PA</state>
                    <description>PENNSYLVANIA</description>
                </statesOfSolicitationList>
                <foreignSolicitation>false</foreignSolicitation>
            </recipient>
        </salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>22530488</totalOfferingAmount>
            <totalAmountSold>4144017</totalAmountSold>
            <totalRemaining>18386471</totalRemaining>
            <clarificationOfResponse></clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>5</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>0</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
            <clarificationOfResponse></clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
            <clarificationOfResponse></clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>AMPCI Macquarie Infrastructure Management No 1 Limited, AMPCI Macquarie Infrastructure Management No 2 Limited, &amp; DUET Investment Holdings Ltd</issuerName>
                <signatureName>Christine Williams</signatureName>
                <nameOfSigner>Christine Williams</nameOfSigner>
                <signatureTitle>Company Secretary</signatureTitle>
                <signatureDate>2011-10-14</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
