<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0306</schemaVersion>

    <documentType>5</documentType>

    <periodOfReport>2018-12-31</periodOfReport>

    <notSubjectToSection16>1</notSubjectToSection16>

    <form3HoldingsReported>1</form3HoldingsReported>

    <form4TransactionsReported>0</form4TransactionsReported>

    <issuer>
        <issuerCik>0001532286</issuerCik>
        <issuerName>Nine Energy Service, Inc.</issuerName>
        <issuerTradingSymbol>NINE</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001728275</rptOwnerCik>
            <rptOwnerName>Woolston Richard S.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>2001 KIRBY DRIVE, SUITE 200</rptOwnerStreet1>
            <rptOwnerStreet2></rptOwnerStreet2>
            <rptOwnerCity>HOUSTON</rptOwnerCity>
            <rptOwnerState>TX</rptOwnerState>
            <rptOwnerZipCode>77019</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>0</isTenPercentOwner>
            <isOther>1</isOther>
            <otherText>See Remarks</otherText>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Common stock</value>
            </securityTitle>
            <transactionCoding>
                <transactionFormType>3</transactionFormType>
                <footnoteId id="F1"/>
            </transactionCoding>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>14608</value>
                    <footnoteId id="F2"/>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">The reporting person owned 192 shares of common stock of the Issuer that were not reported in the reporting person's original Form 3 filed on January 18, 2018 in connection with the effectiveness of the Issuer's Registration Statement on Form S-1 (Registration No. 333-217601). Does not reflect an adjustment for the Issuer's 8.0256-for-1 common stock split that became effective January 23, 2018, in connection with the Issuer's initial public offering.</footnote>
        <footnote id="F2">Reflects adjustment for the Issuer's 8.0256-for-1 common stock split that became effective January 23, 2018, in connection with the Issuer's initial public offering.</footnote>
    </footnotes>

    <remarks>Former Chief Accounting Officer</remarks>

    <ownerSignature>
        <signatureName>/s/ Richard S. Woolston by Theodore R. Moore, as Attorney-in-fact</signatureName>
        <signatureDate>2019-02-13</signatureDate>
    </ownerSignature>
</ownershipDocument>
