<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3</documentType>

    <periodOfReport>2025-07-01</periodOfReport>

    <noSecuritiesOwned>1</noSecuritiesOwned>

    <issuer>
        <issuerCik>0001527469</issuerCik>
        <issuerName>Athene Holding Ltd.</issuerName>
        <issuerTradingSymbol>ATH</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0002076570</rptOwnerCik>
            <rptOwnerName>Brennan Sean Christopher</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O ATHENE HOLDING LTD.</rptOwnerStreet1>
            <rptOwnerStreet2>7700 MILLS CIVIC PKWY</rptOwnerStreet2>
            <rptOwnerCity>WEST DES MOINES</rptOwnerCity>
            <rptOwnerState>IA</rptOwnerState>
            <rptOwnerZipCode>50266</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isOfficer>true</isOfficer>
            <officerTitle>EVP, Chief Commercial Officer</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <remarks>Exhibit 24.1 - Power of Attorney.</remarks>

    <ownerSignature>
        <signatureName>/s/ Ira Rosenblatt, attorney-in-fact</signatureName>
        <signatureDate>2025-07-11</signatureDate>
    </ownerSignature>
</ownershipDocument>
