<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0508</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2025-06-30</periodOfReport>

    <issuer>
        <issuerCik>0001527469</issuerCik>
        <issuerName>Athene Holding Ltd.</issuerName>
        <issuerTradingSymbol>ATH</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001932654</rptOwnerCik>
            <rptOwnerName>Downing Michael Stephen</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O ATHENE HOLDING LTD.</rptOwnerStreet1>
            <rptOwnerStreet2>7700 MILLS CIVIC PKWY</rptOwnerStreet2>
            <rptOwnerCity>WEST DES MOINES</rptOwnerCity>
            <rptOwnerState>IA</rptOwnerState>
            <rptOwnerZipCode>50266</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isOfficer>true</isOfficer>
            <officerTitle>EVP and COO</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <aff10b5One>false</aff10b5One>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Depositary Shares of Series C Preference Stock</value>
                <footnoteId id="F1"/>
            </securityTitle>
            <transactionDate>
                <value>2025-06-30</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>J</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
                <footnoteId id="F2"/>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>8000</value>
                </transactionShares>
                <transactionPricePerShare>
                    <value>25</value>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>D</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>0</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">Complete Title of Security: Depositary Shares, each representing a 1/1,000th interest in a 6.375% Fixed-Rate Reset Perpetual Non-Cumulative Preferred Stock, Series C. The Depositary Shares were not convertible into the shares of Common Stock of the Issuer and did not derive any of their value from the value of the shares of Common Stock of the Issuer and, except in very limited circumstances, were not convertible or exchangeable for any other securities or property of the Issuer and Apollo Global Management, Inc. (&quot;Apollo&quot;), and, except in very limited circumstances, had no voting rights.</footnote>
        <footnote id="F2">These securities were redeemed by the Issuer.</footnote>
    </footnotes>

    <remarks>Exhibit 24.1 - Power of Attorney (incorporated by reference to the Power of Attorney filed as Exhibit 24.1 to the Form 3 filed by the Reporting Person on June 8, 2022)</remarks>

    <ownerSignature>
        <signatureName>/s/ Ira Rosenblatt, attorney-in-fact</signatureName>
        <signatureDate>2025-07-02</signatureDate>
    </ownerSignature>
</ownershipDocument>
