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<edgarSubmission>

    <schemaVersion>X0706</schemaVersion>

    <submissionType>D/A</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001517079</cik>
        <entityName>BLUECROSS BLUESHIELD VENTURES II, INC.</entityName>
        <issuerAddress>
            <street1>225 NORTH MICHIGAN AVENUE</street1>
            <city>CHICAGO</city>
            <stateOrCountry>IL</stateOrCountry>
            <stateOrCountryDescription>ILLINOIS</stateOrCountryDescription>
            <zipCode>60601</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>312-297-6000</issuerPhoneNumber>
        <jurisdictionOfInc>DELAWARE</jurisdictionOfInc>
        <issuerPreviousNameList>
            <value>None</value>
        </issuerPreviousNameList>
        <edgarPreviousNameList>
            <value>None</value>
        </edgarPreviousNameList>
        <entityType>Corporation</entityType>
        <yearOfInc>
            <withinFiveYears>true</withinFiveYears>
            <value>2011</value>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>BYLER</firstName>
                <lastName>KERRY</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>225 NORTH MICHIGAN AVENUE</street1>
                <city>CHICAGO</city>
                <stateOrCountry>IL</stateOrCountry>
                <stateOrCountryDescription>ILLINOIS</stateOrCountryDescription>
                <zipCode>60601</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>ROBERT</firstName>
                <lastName>KOLODGY</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>225 NORTH MICHIGAN AVENUE</street1>
                <city>CHICAGO</city>
                <stateOrCountry>IL</stateOrCountry>
                <stateOrCountryDescription>ILLINOIS</stateOrCountryDescription>
                <zipCode>60601</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>ALAN</firstName>
                <lastName>KRIGSTEIN</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>1901 MARKET STREET</street1>
                <city>PHILADELPHIA</city>
                <stateOrCountry>PA</stateOrCountry>
                <stateOrCountryDescription>PENNSYLVANIA</stateOrCountryDescription>
                <zipCode>19103</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>KWADWO</firstName>
                <middleName>O.</middleName>
                <lastName>DODI</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>THREE PENN PLAZA EAST, PP-15D</street1>
                <city>NEWARK</city>
                <stateOrCountry>NJ</stateOrCountry>
                <stateOrCountryDescription>NEW JERSEY</stateOrCountryDescription>
                <zipCode>07105</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>ROBERT</firstName>
                <middleName>C.</middleName>
                <lastName>JACOBY</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>4800 DEERWOOD CAMPUS PARKWAY, DCC9-1</street1>
                <city>JACKSONVILLE</city>
                <stateOrCountry>FL</stateOrCountry>
                <stateOrCountryDescription>FLORIDA</stateOrCountryDescription>
                <zipCode>32246</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>MICHAEL</firstName>
                <middleName>R.</middleName>
                <lastName>CLEARY</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>2500 ELMERTON AVENUE</street1>
                <city>HARRISBURG</city>
                <stateOrCountry>PA</stateOrCountry>
                <stateOrCountryDescription>PENNSYLVANIA</stateOrCountryDescription>
                <zipCode>17177</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>MARK</firstName>
                <middleName>R.</middleName>
                <lastName>BARTLETT</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>600 EAST LAFAYETTE, MC 21/04</street1>
                <city>DETROIT</city>
                <stateOrCountry>MI</stateOrCountry>
                <stateOrCountryDescription>MICHIGAN</stateOrCountryDescription>
                <zipCode>48226</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>JEANNE</firstName>
                <middleName>A.</middleName>
                <lastName>KENNEDY</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>10455 MILL RUN CIRCLE</street1>
                <city>OWINGS MILLS</city>
                <stateOrCountry>MD</stateOrCountry>
                <stateOrCountryDescription>MARYLAND</stateOrCountryDescription>
                <zipCode>21117</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>CYNTHIA</firstName>
                <middleName>M.</middleName>
                <lastName>VICE</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>450 RIVERCHASE PARKWAY EAST</street1>
                <city>BIRMINGHAM</city>
                <stateOrCountry>AL</stateOrCountry>
                <stateOrCountryDescription>ALABAMA</stateOrCountryDescription>
                <zipCode>35298</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>KEITH</firstName>
                <middleName>F.</middleName>
                <lastName>RENALDI, SR.</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>LANDMARK CENTER</street1>
                <street2>401 PARK DRIVE</street2>
                <city>BOSTON</city>
                <stateOrCountry>MA</stateOrCountry>
                <stateOrCountryDescription>MASSACHUSETTS</stateOrCountryDescription>
                <zipCode>02215</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>JOHN</firstName>
                <lastName>GIBLIN</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>1 CAMERON HILL CIRCLE</street1>
                <city>CHATTANOOGA</city>
                <stateOrCountry>TN</stateOrCountry>
                <stateOrCountryDescription>TENNESSEE</stateOrCountryDescription>
                <zipCode>37402</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>FRANCISCO</firstName>
                <lastName>MARTORELL-BASANTA</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>C/O TRIPLE-S MANAGEMENT CORPORATION</street1>
                <street2>P.O. BOX 363628</street2>
                <city>SAN JUAN</city>
                <stateOrCountry>PR</stateOrCountry>
                <stateOrCountryDescription>PUERTO RICO</stateOrCountryDescription>
                <zipCode>00936</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>CHRISTOPHER</firstName>
                <lastName>LEARDINI</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>257 WEST GENESEE STREET</street1>
                <city>BUFFALO</city>
                <stateOrCountry>NY</stateOrCountry>
                <stateOrCountryDescription>NEW YORK</stateOrCountryDescription>
                <zipCode>14202</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>BRUCE</firstName>
                <middleName>E.</middleName>
                <lastName>SICKEL</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>19 NORTH MAIN STREET</street1>
                <city>WILKES-BARRE</city>
                <stateOrCountry>PA</stateOrCountry>
                <stateOrCountryDescription>PENNSYLVANIA</stateOrCountryDescription>
                <zipCode>18711</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>JACK</firstName>
                <middleName>A.</middleName>
                <lastName>MYERS</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>3000 EAST PINE AVENUE</street1>
                <city>MERIDIAN</city>
                <stateOrCountry>ID</stateOrCountry>
                <stateOrCountryDescription>IDAHO</stateOrCountryDescription>
                <zipCode>83642</zipCode>
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            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>MITCHELL</firstName>
                <middleName>W.</middleName>
                <lastName>PERRY</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>5901 CHAPEL HILL ROAD</street1>
                <city>DURHAM</city>
                <stateOrCountry>NC</stateOrCountry>
                <stateOrCountryDescription>NORTH CAROLINA</stateOrCountryDescription>
                <zipCode>27707</zipCode>
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            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
            <relationshipClarification></relationshipClarification>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>H.F.</firstName>
                <lastName>BEACHAM, III</lastName>
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            <relatedPersonAddress>
                <street1>2 MID AMERICA PLAZA, SUITE 200</street1>
                <city>OAKBROOK TERRACE</city>
                <stateOrCountry>IL</stateOrCountry>
                <stateOrCountryDescription>ILLINOIS</stateOrCountryDescription>
                <zipCode>60181</zipCode>
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        <relatedPersonInfo>
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                <firstName>RONALD</firstName>
                <middleName>D.</middleName>
                <lastName>SIMMONS</lastName>
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            <relatedPersonAddress>
                <street1>1133 SW TOPEKA BOULEVARD</street1>
                <city>TOPEKA</city>
                <stateOrCountry>KS</stateOrCountry>
                <stateOrCountryDescription>KANSAS</stateOrCountryDescription>
                <zipCode>66629</zipCode>
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                <relationship>Director</relationship>
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        <relatedPersonInfo>
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                <firstName>BRYAN</firstName>
                <middleName>R.</middleName>
                <lastName>CAMERLINCK</lastName>
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                <street1>2301 MAIN STREET</street1>
                <city>KANSAS CITY</city>
                <stateOrCountry>MO</stateOrCountry>
                <stateOrCountryDescription>MISSOURI</stateOrCountryDescription>
                <zipCode>64108</zipCode>
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                <relationship>Director</relationship>
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        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>RICHARD</firstName>
                <middleName>F.</middleName>
                <lastName>SCHUM</lastName>
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            <relatedPersonAddress>
                <street1>4000 HOUSE AVENUE</street1>
                <city>CHEYENNE</city>
                <stateOrCountry>WY</stateOrCountry>
                <stateOrCountryDescription>WYOMING</stateOrCountryDescription>
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                <relationship>Director</relationship>
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            <relatedPersonName>
                <firstName>STEVE</firstName>
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                <lastName>RIBBINK</lastName>
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                <street1>818 KEEAUMOKU STREET</street1>
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                <zipCode>96814</zipCode>
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            <relatedPersonName>
                <firstName>KAREN</firstName>
                <lastName>ABRAHAM</lastName>
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            <relatedPersonAddress>
                <street1>8220 N 23RD AVENUE</street1>
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                <zipCode>85021</zipCode>
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                <relationship>Director</relationship>
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        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>NANETTE</firstName>
                <middleName>P.</middleName>
                <lastName>DETURK</lastName>
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            <relatedPersonAddress>
                <street1>120 FIFTH AVENUE</street1>
                <city>PITTSBURGH</city>
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                <stateOrCountryDescription>PENNSYLVANIA</stateOrCountryDescription>
                <zipCode>15222</zipCode>
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        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>TOM</firstName>
                <lastName>BROPHY</lastName>
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            <relatedPersonAddress>
                <street1>50 BEALE STREET</street1>
                <city>SAN FRANCISCO</city>
                <stateOrCountry>CA</stateOrCountry>
                <stateOrCountryDescription>CALIFORNIA</stateOrCountryDescription>
                <zipCode>94105</zipCode>
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    <offeringData>
        <industryGroup>
            <industryGroupType>Pooled Investment Fund</industryGroupType>
            <investmentFundInfo>
                <investmentFundType>Venture Capital Fund</investmentFundType>
                <is40Act>false</is40Act>
            </investmentFundInfo>
        </industryGroup>
        <issuerSize>
            <revenueRange>Decline to Disclose</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06</item>
            <item>3C</item>
            <item>3C.7</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>true</isAmendment>
                <previousAccessionNumber>0001517079-11-000001</previousAccessionNumber>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2011-03-31</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>true</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isEquityType>true</isEquityType>
            <isPooledInvestmentFundType>true</isPooledInvestmentFundType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
            <clarificationOfResponse></clarificationOfResponse>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>20000</minimumInvestmentAccepted>
        <salesCompensationList></salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>1886900</totalOfferingAmount>
            <totalAmountSold>1886900</totalAmountSold>
            <totalRemaining>0</totalRemaining>
            <clarificationOfResponse></clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>23</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>0</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
            <clarificationOfResponse></clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
                <isEstimate>true</isEstimate>
            </grossProceedsUsed>
            <clarificationOfResponse>The investment manager is entitled to a performance fee and a management fee.  The performance fee and management fee are fully discussed in the Issuer's confidential offering materials.</clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>BLUECROSS BLUESHIELD VENTURES II, INC.</issuerName>
                <signatureName>/S/ ROBERT KOLODGY</signatureName>
                <nameOfSigner>ROBERT KOLODGY</nameOfSigner>
                <signatureTitle>PRESIDENT OF THE ISSUER</signatureTitle>
                <signatureDate>2013-08-30</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
