<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3</documentType>

    <periodOfReport>2024-07-30</periodOfReport>

    <noSecuritiesOwned>0</noSecuritiesOwned>

    <issuer>
        <issuerCik>0001506983</issuerCik>
        <issuerName>Glucotrack, Inc.</issuerName>
        <issuerTradingSymbol>GCTK</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0002046615</rptOwnerCik>
            <rptOwnerName>John A. Ballantyne Revocable Living Trust dated 08/01/2017</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>7410 CLAIRE DRIVE SOUTH</rptOwnerStreet1>
            <rptOwnerStreet2></rptOwnerStreet2>
            <rptOwnerCity>FARGO</rptOwnerCity>
            <rptOwnerState>ND</rptOwnerState>
            <rptOwnerZipCode>58104</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Common Stock, par value $0.001 per share</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>51002</value>
                    <footnoteId id="F1"/>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">John A. Ballantyne is the trustee of the reporting person and has sole voting and investment power over all securities owned by the reporting person.</footnote>
    </footnotes>

    <remarks>Exhibit List:
Exhibit 24 - Power of Attorney

Note: On May 17, 2024, a 1-for-5 reverse stock split of the Issuer's common stock, par value $0.001 per share (the &quot;Common Stock&quot;) was implemented (the &quot;2024 Reverse Split&quot;). On February 25, 2025, a 1-for-20 reverse stock split of the Common Stock was implemented (the &quot;2025 Reverse Split,&quot; and together with the 2024 Reverse Split, the &quot;Reverse Stock Splits&quot;). All figures presented in this Form 3 reflect the Reverse Stock Splits.</remarks>

    <ownerSignature>
        <signatureName>/s/ Nelson Mullins Riley &amp; Scarborough LLP, Attorney-in-Fact</signatureName>
        <signatureDate>2025-03-28</signatureDate>
    </ownerSignature>
</ownershipDocument>
