SCHEDULE 13G/A 0001930214 XXXXXXXX LIVE 1 Common Stock, $0.001 par value 03/31/2025 0001505512 Regulus Therapeutics Inc. 75915K309 4224 Campus Point Court Suite 210 San Diego CA 92121 Rule 13d-1(c) Vivo Opportunity Fund Holdings, L.P. b DE 2696426.00 0.00 2696426.00 0.00 2696426.00 N 3.9 PN (1) The number represents shares of common stock, $0.001 par value (the "Common Stock") of Regulus Therapeutics Inc. (the "Issuer") held of record by Vivo Opportunity Fund Holdings, L.P. Vivo Opportunity, LLC is the general partner of Vivo Opportunity Fund Holdings, L.P. (2) Based on 69,234,089 shares of Common Stock outstanding as of May 2, 2025, as disclosed in the Issuer's Quarterly Report on Form 10-Q, filed with the Securities and Exchange Commission (the "SEC") on May 8, 2025. Vivo Opportunity, LLC b DE 2696426.00 0.00 2696426.00 0.00 2696426.00 N 3.9 OO (1) The number represents shares of Common Stock of the Issuer held of record by Vivo Opportunity Fund Holdings, L.P. Vivo Opportunity, LLC is the general partner of Vivo Opportunity Fund Holdings, L.P. (2) Based on 69,234,089 shares of Common Stock outstanding as of May 2, 2025, as disclosed in the Issuer's Quarterly Report on Form 10-Q, filed with the SEC on May 8, 2025. Regulus Therapeutics Inc. 4224 Campus Point Court, Suite 210 San Diego, CA 92121 (i) Vivo Opportunity Fund Holdings, L.P. (ii) Vivo Opportunity, LLC 192 Lytton Avenue, Palo Alto, CA 94301 (i) Vivo Opportunity Fund Holdings, L.P., a Delaware limited partnership; and (ii) Vivo Opportunity, LLC, a Delaware limited liability company. Vivo Opportunity, LLC is the general partner of Vivo Opportunity Fund Holdings, L.P. Y All securities are held of record by Vivo Opportunity Fund Holdings, L.P. Vivo Opportunity, LLC is the general partner of Vivo Opportunity Fund Holdings, L.P. 3.9% 2,696,426 0 2,696,426 0 N Y Y Y Y Y N By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under ?? 240.14a-11. Vivo Opportunity Fund Holdings, L.P. /s/ Kevin Dai 05/14/2025 Vivo Opportunity, LLC /s/ Kevin Dai 05/14/2025