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        <fairValLevel>1</fairValLevel>
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          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Danaher Corp.</name>
        <lei>S4BKK9OTCEWQ3YHPFM11</lei>
        <title>Danaher Corp.</title>
        <cusip>235851102</cusip>
        <identifiers>
          <isin value="US2358511028"/>
        </identifiers>
        <balance>3959.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>866585.51000000</valUSD>
        <pctVal>2.543481623907</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Howmet Aerospace, Inc.</name>
        <lei>549300HO5WFZUT5N2T22</lei>
        <title>Howmet Aerospace, Inc.</title>
        <cusip>443201108</cusip>
        <identifiers>
          <isin value="US4432011082"/>
        </identifiers>
        <balance>7967.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>1657773.36000000</valUSD>
        <pctVal>4.865666491196</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Cash Collateral Fund LLC</name>
        <lei>N/A</lei>
        <title>Janus Henderson Cash Collateral Fund LLC</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Custodian Internal ID" value="191217031"/>
        </identifiers>
        <balance>360282.00000000</balance>
        <units>PA</units>
        <curCd>USD</curCd>
        <valUSD>360282.00000000</valUSD>
        <pctVal>1.057449767911</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>STIV</assetCat>
        <issuerConditional desc="Non-registered Fund" issuerCat="OTHER"/>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <debtSec>
          <maturityDt>2026-02-02</maturityDt>
          <couponKind>Fixed</couponKind>
          <annualizedRt>3.59890000</annualizedRt>
          <isDefault>N</isDefault>
          <areIntrstPmntsInArrs>N</areIntrstPmntsInArrs>
          <isPaidKind>N</isPaidKind>
        </debtSec>
        <securityLending>
          <cashCollateralCondition cashCollateralVal="360282.00000000" isCashCollateral="Y"/>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Nebius Group NV</name>
        <lei>549300GGZHJ5Q1B18P93</lei>
        <title>Nebius Group NV, Class A</title>
        <cusip>N/A</cusip>
        <identifiers>
          <isin value="NL0009805522"/>
        </identifiers>
        <balance>9042.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>770287.98000000</valUSD>
        <pctVal>2.260842466944</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>NL</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <loanByFundCondition isLoanByFund="Y" loanVal="441331.50000000"/>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>DoorDash, Inc.</name>
        <lei>549300NUQ43FGGSK5051</lei>
        <title>DoorDash, Inc., Class A</title>
        <cusip>25809K105</cusip>
        <identifiers>
          <isin value="US25809K1051"/>
        </identifiers>
        <balance>4223.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>864110.26000000</valUSD>
        <pctVal>2.536216613337</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Boeing Co. (The)</name>
        <lei>RVHJWBXLJ1RFUBSY1F30</lei>
        <title>Boeing Co. (The)</title>
        <cusip>097023105</cusip>
        <identifiers>
          <isin value="US0970231058"/>
        </identifiers>
        <balance>6938.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>1621549.36000000</valUSD>
        <pctVal>4.759346829395</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Revolution Medicines, Inc.</name>
        <lei>549300JU3X0LBCQ65892</lei>
        <title>Revolution Medicines, Inc.</title>
        <cusip>76155X100</cusip>
        <identifiers>
          <isin value="US76155X1000"/>
        </identifiers>
        <balance>5326.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>516355.70000000</valUSD>
        <pctVal>1.515535650197</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>DraftKings, Inc.</name>
        <lei>2549006G33IUL6IVOD83</lei>
        <title>DraftKings, Inc., Class A</title>
        <cusip>26142V105</cusip>
        <identifiers>
          <isin value="US26142V1052"/>
        </identifiers>
        <balance>50475.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>1388567.25000000</valUSD>
        <pctVal>4.075530046578</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>PTC, Inc.</name>
        <lei>54930050SSMU8SKDJ030</lei>
        <title>PTC, Inc.</title>
        <cusip>69370C100</cusip>
        <identifiers>
          <isin value="US69370C1009"/>
        </identifiers>
        <balance>3549.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>554105.37000000</valUSD>
        <pctVal>1.626333247025</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Legence Corp.</name>
        <lei>N/A</lei>
        <title>Legence Corp., Class A</title>
        <cusip>52476L109</cusip>
        <identifiers>
          <isin value="US52476L1098"/>
        </identifiers>
        <balance>14894.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>698677.54000000</valUSD>
        <pctVal>2.050661433315</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Chipotle Mexican Grill, Inc.</name>
        <lei>529900REP5VGTPCP1J71</lei>
        <title>Chipotle Mexican Grill, Inc., Class A</title>
        <cusip>169656105</cusip>
        <identifiers>
          <isin value="US1696561059"/>
        </identifiers>
        <balance>23054.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>896108.98000000</valUSD>
        <pctVal>2.630134819180</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Broadcom, Inc.</name>
        <lei>549300WV6GIDOZJTV909</lei>
        <title>Broadcom, Inc.</title>
        <cusip>11135F101</cusip>
        <identifiers>
          <isin value="US11135F1012"/>
        </identifiers>
        <balance>7976.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>2642448.80000000</valUSD>
        <pctVal>7.755749302704</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Datadog, Inc.</name>
        <lei>549300F6JNO0KRPO1K63</lei>
        <title>Datadog, Inc., Class A</title>
        <cusip>23804L103</cusip>
        <identifiers>
          <isin value="US23804L1035"/>
        </identifiers>
        <balance>10244.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>1324754.08000000</valUSD>
        <pctVal>3.888234478645</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Vaxcyte, Inc.</name>
        <lei>254900M7NMHTGASX8D95</lei>
        <title>Vaxcyte, Inc.</title>
        <cusip>92243G108</cusip>
        <identifiers>
          <isin value="US92243G1085"/>
        </identifiers>
        <balance>9545.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>511325.65000000</valUSD>
        <pctVal>1.500772144929</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>ROYAL BANK OF CANADA</name>
        <lei>ES7IP3U3RHIGC71XBU11</lei>
        <title>Other Note or Other Instrument</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Custodian Internal ID" value="260130018"/>
        </identifiers>
        <balance>90070.50000000</balance>
        <units>PA</units>
        <curCd>USD</curCd>
        <valUSD>90070.50000000</valUSD>
        <pctVal>0.264362441977</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>STIV</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>CA</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <debtSec>
          <maturityDt>2026-02-02</maturityDt>
          <couponKind>Fixed</couponKind>
          <annualizedRt>3.65000000</annualizedRt>
          <isDefault>N</isDefault>
          <areIntrstPmntsInArrs>N</areIntrstPmntsInArrs>
          <isPaidKind>N</isPaidKind>
        </debtSec>
        <securityLending>
          <cashCollateralCondition cashCollateralVal="90070.50000000" isCashCollateral="Y"/>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Cash Liquidity Fund LLC</name>
        <lei>N/A</lei>
        <title>Janus Henderson Cash Liquidity Fund LLC</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Inhouse Asset ID" value="ABH9984U2"/>
        </identifiers>
        <balance>187411.32000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>187467.54000000</valUSD>
        <pctVal>0.550228728229</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>STIV</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>AppLovin Corp.</name>
        <lei>549300LLVXMUAOL3SQ07</lei>
        <title>AppLovin Corp., Class A</title>
        <cusip>03831W108</cusip>
        <identifiers>
          <isin value="US03831W1080"/>
        </identifiers>
        <balance>1545.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>730954.95000000</valUSD>
        <pctVal>2.145397611401</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>MercadoLibre, Inc.</name>
        <lei>549300DKPDN9M5S8GB14</lei>
        <title>MercadoLibre, Inc.</title>
        <cusip>58733R102</cusip>
        <identifiers>
          <isin value="US58733R1023"/>
        </identifiers>
        <balance>639.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>1372437.81000000</valUSD>
        <pctVal>4.028189150878</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>

        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Shopify, Inc.</name>
        <lei>549300HGQ43STJLLP808</lei>
        <title>Shopify, Inc., Class A</title>
        <cusip>82509L107</cusip>
        <identifiers>
          <isin value="CA82509L1076"/>
        </identifiers>
        <balance>5105.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>669929.15000000</valUSD>
        <pctVal>1.966283145381</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>CA</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Booking Holdings, Inc.</name>
        <lei>FXM8FAOHMYDIPD38UZ17</lei>
        <title>Booking Holdings, Inc.</title>
        <cusip>09857L108</cusip>
        <identifiers>
          <isin value="US09857L1089"/>
        </identifiers>
        <balance>228.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>1140419.52000000</valUSD>
        <pctVal>3.347201238877</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>UnitedHealth Group, Inc.</name>
        <lei>549300GHBMY8T5GXDE41</lei>
        <title>UnitedHealth Group, Inc.</title>
        <cusip>91324P102</cusip>
        <identifiers>
          <isin value="US91324P1021"/>
        </identifiers>
        <balance>3335.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>956911.55000000</valUSD>
        <pctVal>2.808594091458</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>

        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
    </invstOrSecs>
    <explntrNotes>
      <explntrNote note="Monthly returns presented in Item B.5(a) have been calculated without deducting any applicable sales loads or redemption fees." noteItem="B.5.a"/>
    </explntrNotes>
    <signature>
      <ncom:dateSigned>2026-03-02</ncom:dateSigned>
      <ncom:nameOfApplicant>Janus Detroit Street Trust</ncom:nameOfApplicant>
      <ncom:signature>Jesper Nergaard</ncom:signature>
      <ncom:signerName>Jesper Nergaard</ncom:signerName>
      <ncom:title>VP, CFO, Treasurer, and Principal Accounting Officer</ncom:title>
    </signature>
  </formData>
  <documents>XXXX</documents>
</edgarSubmission>
