[ Janus Henderson Letterhead ]

August 16, 2024

EDGAR Operations Branch

Securities and Exchange Commission

Division of Investment Management

100 F Street, N.E.

Washington, D.C. 20549-0505

Re: JANUS DETROIT STREET TRUST (the “Registrant”)

1933 Act File No. 333-207814

1940 Act File No. 811-23112

Dear Sir or Madam:

Pursuant to Rule 497(j) of Regulation C under the Securities Act of 1933, as amended (the “1933 Act”), the undersigned hereby certifies on behalf of the Registrant:

1. There are no changes to the Statement of Additional Information from the form of the Statement of Additional Information that was filed in Post-Effective Amendment No. 58 (“PEA No. 58”) on August 12, 2024, pursuant to Rule 485(b) under the 1933 Act for Janus Henderson Emerging Markets Debt Hard Currency ETF.

2. The text of PEA No. 58 has been filed electronically.

If you have any questions regarding this filing, please call me at (303) 336-7903.

Respectfully,

/s/ Cara Owen

Cara Owen, Esq.

Vice President, Chief Legal Officer, and Secretary of the Registrant

Enclosure (via EDGAR)

cc: Eric S. Purple, Esq.