<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0704</schemaVersion>

    <submissionType>D</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001494650</cik>
        <entityName>OptiNose, Inc.</entityName>
        <issuerAddress>
            <street1>WFD Ventures LLC</street1>
            <street2>152 West 57th Street, 10th Floor</street2>
            <city>New York</city>
            <stateOrCountry>NY</stateOrCountry>
            <stateOrCountryDescription>NEW YORK</stateOrCountryDescription>
            <zipCode>10019</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>(212) 527-5787</issuerPhoneNumber>
        <jurisdictionOfInc>DELAWARE</jurisdictionOfInc>
        <issuerPreviousNameList>
            <value>None</value>
        </issuerPreviousNameList>
        <edgarPreviousNameList>
            <value>None</value>
        </edgarPreviousNameList>
        <entityType>Corporation</entityType>
        <yearOfInc>
            <withinFiveYears>true</withinFiveYears>
            <value>2010</value>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Peter</firstName>
                <lastName>Miller</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>OptiNose, Inc.</street1>
                <street2>WFD Ventures LLC, 152 West 57th St.</street2>
                <city>New York</city>
                <stateOrCountry>NY</stateOrCountry>
                <stateOrCountryDescription>NEW YORK</stateOrCountryDescription>
                <zipCode>10019</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Helena</firstName>
                <middleName>Kyttari</middleName>
                <lastName>Djupesland</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>OptiNose AS, Oslo Innovation Centre</street1>
                <street2>Gaustadalleen 21, 0349</street2>
                <city>Oslo</city>
                <stateOrCountry>Q8</stateOrCountry>
                <stateOrCountryDescription>NORWAY</stateOrCountryDescription>
                <zipCode>N-0349</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Ramy</firstName>
                <lastName>Mahmoud</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>WFD Ventures LLC</street1>
                <street2>152 West 57th St., 10th Floor</street2>
                <city>New York</city>
                <stateOrCountry>NY</stateOrCountry>
                <stateOrCountryDescription>NEW YORK</stateOrCountryDescription>
                <zipCode>10019</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Henrik</firstName>
                <middleName>Bruun</middleName>
                <lastName>Andersen</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>OptiNose AS, Oslo Innovation Centre</street1>
                <street2>Gaustadalleen 21, 0349</street2>
                <city>Oslo</city>
                <stateOrCountry>Q8</stateOrCountry>
                <stateOrCountryDescription>NORWAY</stateOrCountryDescription>
                <zipCode>N-0349</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Per</firstName>
                <lastName>Djupesland</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>OptiNose AS, Oslo Innovation Centre</street1>
                <street2>Gaustadalleen 21, 0349</street2>
                <city>Oslo</city>
                <stateOrCountry>Q8</stateOrCountry>
                <stateOrCountryDescription>NORWAY</stateOrCountryDescription>
                <zipCode>N-0349</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Klaas</firstName>
                <lastName>de Boer</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>Entrepreneurs Fund LP</street1>
                <street2>Windward House-La Route de la Liberation</street2>
                <city>St. Helier</city>
                <stateOrCountry>Y9</stateOrCountry>
                <stateOrCountryDescription>JERSEY</stateOrCountryDescription>
                <zipCode>JE23BQ</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>William</firstName>
                <middleName>F.</middleName>
                <lastName>Doyle</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>WFD Ventures LLC</street1>
                <street2>152 West 57th St., 10th Floor</street2>
                <city>New York</city>
                <stateOrCountry>NY</stateOrCountry>
                <stateOrCountryDescription>NEW YORK</stateOrCountryDescription>
                <zipCode>10019</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Larry</firstName>
                <lastName>Pickering</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>Avista Capital Holdings, LP</street1>
                <street2>65 E. 55th Street, 18th Floor</street2>
                <city>New York</city>
                <stateOrCountry>NY</stateOrCountry>
                <stateOrCountryDescription>NEW YORK</stateOrCountryDescription>
                <zipCode>10022</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Patrick</firstName>
                <lastName>O'Neill</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>Avista Capital Holdings, LP</street1>
                <street2>65 E. 55th Street, 18th Floor</street2>
                <city>New York</city>
                <stateOrCountry>NY</stateOrCountry>
                <stateOrCountryDescription>NEW YORK</stateOrCountryDescription>
                <zipCode>10022</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Sriram</firstName>
                <lastName>Venkataraman</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>Avista Capital Holdings, LP</street1>
                <street2>65 E. 55th Street, 18th Floor</street2>
                <city>New York</city>
                <stateOrCountry>NY</stateOrCountry>
                <stateOrCountryDescription>NEW YORK</stateOrCountryDescription>
                <zipCode>10022</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Other Health Care</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>Decline to Disclose</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>false</isAmendment>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2010-06-07</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>true</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isEquityType>true</isEquityType>
            <isOptionToAcquireType>true</isOptionToAcquireType>
            <isSecurityToBeAcquiredType>true</isSecurityToBeAcquiredType>
            <isOtherType>true</isOtherType>
            <descriptionOfOtherType>Common Stock; Series A, B-1, B-2 and C Preferred Stock; Common Stock Warrants</descriptionOfOtherType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>true</isBusinessCombinationTransaction>
            <clarificationOfResponse>A private offering of Series C Preferred Stock and Common Stock is being made in connection with, and subsequent to, a security-for-security exchange in the formation of the issuer.</clarificationOfResponse>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>0</minimumInvestmentAccepted>
        <salesCompensationList></salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>48750011</totalOfferingAmount>
            <totalAmountSold>21055999</totalAmountSold>
            <totalRemaining>27694012</totalRemaining>
            <clarificationOfResponse>This amount does not include the FMV of the securities issued in the exchange.</clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>23</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>0</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>OptiNose, Inc.</issuerName>
                <signatureName>/s/ Peter Miller</signatureName>
                <nameOfSigner>Peter Miller</nameOfSigner>
                <signatureTitle>President and Chief Executive Officer</signatureTitle>
                <signatureDate>2010-06-18</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
