SCHEDULE 13G/A 0000950170-25-072011 0001493215 XXXXXXXX LIVE 2 Ordinary Shares, par value 0.05 euro 09/30/2025 0001590560 uniQure N.V. N90064101 Paasheuvelweg 25a Amsterdam P7 1105 BP Rule 13d-1(b) RTW Investments, LP DE 0.00 4424968.00 0.00 4424968.00 4424968.00 7.2 IA PN Roderick Wong X1 0.00 4424968.00 0.00 4424968.00 4424968.00 7.2 HC IN uniQure N.V. Paasheuvelweg 25a, Amsterdam, P7, 1105 BP. This statement is filed by: (i) RTW Investments, LP ("RTW Investments"), a Delaware limited partnership and the investment adviser to certain funds (the "RTW Funds"), with respect to the Ordinary Shares, par value Euro 0.05 (the "Shares") of uniQure N.V. (the "Company") directly held by the RTW Funds; and (ii) Roderick Wong, M.D. ("Dr. Wong"), the Managing Partner and Chief Investment Officer of RTW Investments, with respect to the Shares directly held by the RTW Funds. The foregoing persons are hereinafter sometimes collectively referred to as the "Reporting Persons." The filing of this statement should not be construed as an admission that any of the foregoing persons or any Reporting Person is, for the purposes of Section 13 of the Securities Exchange Act of 1934, as amended, the beneficial owner of the Shares reported herein. The address of the business office of each of the Reporting Persons is 40 10th Avenue, Floor 7, New York, New York 10014. RTW Investments is a Delaware limited partnership. Dr. Wong is a citizen of the United States. N IA HC The information required by Item 4(a) is set forth in Rows 5 - 9 of the cover page for each of the Reporting Persons and is incorporated herein by reference. The percentages set forth in Row 11 of the cover pages are calculated based upon 54,866,930 Shares outstanding as of July 24, 2025 as reported in the Issuer's Quarterly Report on Form 10-Q filed with the Securities and Exchange Commission (the "SEC") on July 29, 2025, plus 6,736,841 Shares issued pursuant to the Issuer's public offering that closed on August 15, 2025, as disclosed in the prospectus supplement dated September 25, 2025 and filed with the SEC on September 29, 2025. RTW Investments: 7.2% Dr. Wong: 7.2% RTW Investments: 0 Dr. Wong: 0 RTW Investments: 4,424,968 Shares Dr. Wong: 4,424,968 Shares RTW Investments: 0 Dr. Wong: 0 RTW Investments: 4,424,968 Shares Dr. Wong: 4,424,968 Shares Y N See Item 2. The RTW Funds have the right to receive or the power to direct the receipt of dividends from, or the proceeds from the sale of, the Shares reported herein. Y Y Y N By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were acquired and are held in the ordinary course of business and were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under ?? 240.14a-11. Exhibit 99.1 Joint Filing Agreement (incorporated by reference to Exhibit 99.1 to the Reporting Persons' Schedule 13G filed with the SEC on May 15, 2025). RTW Investments, LP /s/ Roderick Wong, M.D. 11/14/2025 Roderick Wong s/ Roderick Wong, M.D. 11/14/2025