SCHEDULE 13G/A 0001193805-24-001310 0001381740 XXXXXXXX LIVE 1 Common Stock, par value $0.001 per share 12/31/2024 0001492674 T2 Biosystems, Inc. 89853L302 101 HARTWELL AVENUE LEXINGTON MA 02421 Rule 13d-1(c) Safier Jacob X1 1500000.00 0.00 1500000.00 0.00 1500000.00 N 5.3 IN JSAF Holdings, LLC DE 1500000.00 0.00 1500000.00 0.00 1500000.00 N 5.3 OO T2 Biosystems, Inc. 101 Hartwell Ave., Lexington, MA 02421 JSAF Holdings, LLC ("JSAF") Jacob Safier Each of the foregoing is referred to as a "Reporting Person" and collectively as the "Reporting Persons." JSAF Holdings, LLC c/o The Wolfson Group One State Street Plaza, 29th Floor New York, NY 10004 Jacob Safier c/o The Wolfson Group One State Street Plaza, 29th Floor New York, NY 10004 JSAF Holdings, LLC Delaware Jacob Safier United States Y As of the date hereof: JSAF beneficially owns 1,500,000 Shares. Jacob Safier, as the manager of the T2 Biosystems, Inc. investment by JSAF, may be deemed to beneficially own the Shares owned by JSAF and, therefore, may be deemed to beneficially own 1,500,000 Shares. Mr. Safier disclaims beneficial ownership of the Shares owned by JSAF. As of the date hereof, JSAF beneficially owned and Jacob Safier may be deemed to beneficially own 5.3% (based upon 28,045,417 Shares outstanding, which is the number of Shares reported as outstanding in the Issuer's Proxy Statement on Schedule 14-A filed with the Securities and Exchange Commission on January 21, 2025). 1,500,000 Shares. 0 Shares. 1,500,000 Shares. 0 Shares. Y Y Y Y Y N By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under ?? 240.14a-11. Safier Jacob /s/ Jacob Safier 02/13/2025 JSAF Holdings, LLC /s/ Jacob Safier 02/13/2025